Type I Collagen Grafting on Titanium Surfaces Using Low-temperature Glow Discharge

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概要

• 論文の詳細を見る

• To improve the bioactivity of titanium surfaces, glow discharge was used to facilitate collagen grafting on titanium disks. Titanium test specimens were pre-treated by glow discharge fed with a mixture of argon and allylamine (AA) gases. Treated titanium disks were then grafted with type I collagen using glutaraldehyde (GA) as a crosslinking agent. The surfaces of collagen-grafted titanium disks were evaluated using scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) and X-ray photoelectron spectroscopy (XPS). MG-63 osteoblast-like cells were cultured on the grafted titanium surfaces to examine the effect of collagen grafting in terms of cell morphology. Our results demonstrated that collagen component elements could be detected on the titanium surfaces. Morphology of the cells on the surfaces of collagen-grafted titanium disks indicated differentiation. These findings showed that type I collagen could be successfully grafted onto titanium surfaces using glow discharge technology, with enhanced biofunctionality demonstrated on osteoblastic cells.

• 日本歯科理工学会の論文

著者

• ABIKO Yoshimitsu

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• CHANG Wei-Jen

  School of Dentistry, College of Oral Medicine, Taipei Medical University

• LEE Sheng-Yang

  School of Dentistry, College of Oral Medicine, Taipei Medical University

• Lee Sheng-yang

  School Of Dentistry Taipei Medical University

• Chang Wei-jen

  School Of Oral Hygiene Taipei Medical University

• Abiko Yoshimitsu

  Department Of Biochemistry Nihon University School Dentistry At Matsudo

• Chen Jin-yu

  School Of Dentistry Taipei Medical University

• Lin Che-tong

  School Of Dentistry Taipei Medical University

• OU Keng-Liang

  Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University

• HUANG Haw-Ming

  Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University

• Ou Keng-liang

  Graduate Institute Of Biomedical Materials And Engineering Taipei Medical University

• Huang Haw-ming

  Graduate Institute Of Biomedical Materials And Engineering Taipei Medical University

• Abiko Y

  Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo:res

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Chang Wei-jen

  School Of Dentistry College Of Oral Medicine Taipei Medical University

• Lee Sheng-yang

  School Of Dentistry College Of Oral Medicine Taipei Medical University

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