Effect of oxygen radical on apoptosis in human submandibular gland cell lines

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概要

• 論文の詳細を見る

• Oxygen radicals have been implicated as being involved in cellular damage, oxidation of proteins, membrane lipid peroxidation, inactivation of enzymes, and DNA chain breakage during the processes of aging, inflammation, and injury. Apoptosis is an essential mechanism for the selective elimination of cells that is associated with a variety of disorders and encompasses most cell systems. We investigated the effects of extracellular oxygen radicals on apoptosis in a cultured human submandibular gland (HSG) cell line and HSG-AZA3 cells, using an acinar cell phenotype that was induced by treatment of HSG cells with 5-azacytidine. The induction of apoptosis was examined after the cells were exposed to an oxygen radical system composed of hypoxanthine (HX) and xanthine oxidase (XOD). The appearance of nuclei with DNA fragmentation was detected by a TUNEL method while the expression of active casepase-3 was found by immunocytochemistry. Apoptosis of HSG and HSG-AZA3 cells induced with HX/XOD was significantly greater, and active caspase-3 staining intensity was greater than in the control. These findings suggest that caspase-3 expression may be regulated by oxygen radicals and involved in oxygen radical-induced apoptosis of HSG and HSG-AZA3 cells. Moreover, these established cell lines are useful as a model of aging in submandibular glands.

• 日本大学の論文

著者

• ABIKO Yoshimitsu

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Tamaki Hiroyuki

  Department Of Physiological Sciences National Institute Of Fitness And Sports

• Tamaki Hiroyuki

  Department Of Cardiology Mimihara General Hospital

• Tamaki Hiroyuki

  Departments Of Oral Surgery Nihon University School Of Dentistry At Matsudo

• Kamino Yoshikazu

  Departments of Oral surgery, Nihon University School of Dentistry at Matsudo

• Nagura Hideaki

  Departments of Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

• Abiko Yoshimitsu

  Departments Of Biochemistry Nihon University School Of Dentistry At Matsudo

• Nagura Hideaki

  Departments Of Research Institute Of Oral Science Nihon University School Of Dentistry At Matsudo

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Kamino Yoshikazu

  Department Of Maxillofacial Surgery Nihon University School Of Dentistry At Matsudo

• Kamino Yoshikazu

  Departments Of Oral Surgery Nihon University School Of Dentistry At Matsudo

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