Differentiation of the human mesenchymal stem cells derived from bone marrow and enhancement of cell attachment by fibronectin
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概要
- 論文の詳細を見る
The ability of human mesenchymal stem cells (hMSC) to differentiate into osteoblasts was examined through the use of osteogenic induction medium (MSCOIM) cultures. hMSC first attached to the dish surface and exhibited fibroblast-like spindle shapes, and after proliferation, formed cuboidal shapes. Calcium assays and the use of von Kossa and alizarin red S staining showed that hMSC were capable of mineralization when cultured in MSCOIM. Gene expressions of Cbfa-1 and BMP-4, which are markers for osteogenic differentiation, were also increased during the hMSC differentiation into osteoblasts. When compared to albumin (Alb)-coated dishes, microscopic observation documented enhanced cell attachment and spreading when hMSC were cultured on fibronectin (FN)-coated dishes. Adherent cell numbers also exhibited a greater increase on the FN-coated dishes during earlier culture stages than that seen for the Alb-coated dishes. These findings suggest that hMSC have the capability to differentiate into osteoblasts and that FN can stimulate the attachment and spreading of the hMSC. (J. Oral Sci. 46, 207-213, 2004)
- 日本大学歯学部の論文
著者
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Lee Sheng-yang
Graduate Institute Of Oral Rehabilitation Sciences Collage Of Oral Medicine Taipei Medical Universit
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Kondoh Toshirou
Department Of Maxillofacial Surgery Nihon University School Of Dentistry At Matsudo
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Abiko Yoshimitsu
Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud
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Zhang Qi
Wuhan University, School of Stomatology
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Kawada Masaru
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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Ogura Naomi
Department of Oral Surgery, Nihon University School of Dentistry at Matsudo
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Ogura Naomi
Department of Maxillofacial Surgery
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Chang Wei-Jen
Department of Biochemistry, Nihon University School of Dentistry at Matsudo
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Lee Sheng-Yang
Graduate Institute of Oral Rehabilitation Sciences and School of Dentistry, Taipei Medical University
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Kondoh Toshirou
Department of Maxillofacial Surgery
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