Comparative Analysis of MicroRNA-mRNA Expression Profiles of Mesenchymal Stem Cells and Dental Follicle Cells
スポンサーリンク
概要
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Small non-coding microRNAs (miRNAs) regulate cellular and developmental processes through the modulation oftarget gene expression and/or protein synthesis. miRNAs are expressed in a cell-specific manner. Human mesenchymal bone marrow stem cells (hMSC) and human dental follicle cells (hDFC) can undergo multilineage differentiation into various connective tissue cell types, and may therefore be utilized for regeneration in cell therapy. To elucidate the molecular differences that underlie the more primitive characteristics ofhMSC and hDFC, we performed a comprehensive comparative analysis ofthe miRNA and mRNA expression profiles ofhMSC and hDFC using a two-fold change in the normalized intensity of expression as a cut-off to determine differences in expression. In hMSC, 37 were more highly expressed, while 32 miRNAs more highly expressed in hDFC. hMSC expressed high levels ofmiR-196a, -196b, and miR-10a, -10b, which target several homeobox (HOX) genes associated with the patterning ofthe vertebrate axial skeleton. hMSC, but not hDFC, expressed high mRNA levels ofHOX genes. In contrast, miR-199b, which is expressed in tooth germ, as well as the forkhead box O1 (FOXO1) gene, a target ofmiR-1271, were more highly expressed in DFC than in hMSC. The FOXO1 transcription factor plays a role in tooth development and regulates biomineralization. These miRNAs showed specific expression in tissues from which stem cells originate, suggesting that they influence the characteristic mRNA expression of these tissues. These data suggest that analysis of miRNA-mRNA expression profiles is useful for characterization of specific tissue types and cell-specific markers.
著者
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Ogura Naomi
Department Of Maxillofacial Surgery Nihon University School Of Dentistry At Matsudo
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Kondoh Toshirou
Department Of Maxillofacial Surgery Nihon University School Of Dentistry At Matsudo
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Hashimoto Hiroshi
Department Of Adult Disease Jr Tokyo General Hospital
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Takahashi Kosuke
Department of Cardiology, Shizuoka City Shizuoka Hospital
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Iwai Satoshi
Department of Maxillofacial Surgery
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Fujimoto Yoko
Department of Maxillofacial Surgery
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Ogura Naomi
Department of Maxillofacial Surgery
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Kondoh Toshirou
Department of Maxillofacial Surgery
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