Monitoring of dnaK gene expression in Porphyromonas gingivalis by oxygen stress using DNA microarray

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概要

• 論文の詳細を見る

• Porphyromonas gingivalis, a Gram-negative anaerobe associated with adult periodontitis, expresses numerous potential virulence factors. dnaK, a member of the heat shock protein family, functions as a molecular chaperone and plays a role in microbial pathogenicity. However, little is known regarding its gene expression caused by oxygen stress in P. gingivalis. In the present study, a custom-made DNA microarray was designed and used to monitor dnaK gene expression in P. gingivalis caused by oxygen stress. The results demonstrated that dnaK mRNA was up-regulated in a short time, and the DNA microarray results were confirmed by real-time polymerase chain reaction analysis. These findings suggest that oxygen stress stimulates gene expression of dnaK and may have a relationship to the aerotolerance activity of this organism as well as its expression of pathogenesis. (J. Oral Sci. 46, 93-100, 2004)

• 日本大学歯学部の論文

著者

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Araki Makoto

  Department Of Applied Electronics Tokyo University Of Science

• Hiratsuka Koichi

  Department Of Surgery I Oita Medical University

• Kiyama-kishikawa Michiko

  Department Of Biochemistry And Research Institute Of Oral Science Nihon University School Of Dentist

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