T Helper Cytokine Responses Elicited by Nasal Immunization with Heat-Killed Recombinant Lactobacillus casei Expressing Outer Membrane Protein of Porphyromonas gingivalis
スポンサーリンク
概要
- 論文の詳細を見る
Our previous study have demonstrated that nasal administration of heat-killed recombinant Lactobacillus casei expressing 40-kDa outer membrane protein of Porphyromonas gingivalis (L. casei/40k-OMP) elicited high titers of 40k-OMP-specific serum IgG, IgA and salivary IgA antibody responses which provided significant protection against oral infection with P. gingivalis. In this study, we further elucidated nature of 40k-OMP-specific CD4+ T helper (Th) cells in those immunized mice. When CD4+ T cells that were isolated from spleen or cervical lymph nodes of mice immunized with heat-killed L. casei/40k-OMP were restimulated with 40k-OMP in vitro, significant levels of proliferation occurred. Furthermore, heat-killed L. casei/40k-OMP induced 40k-OMP-specific Th1 cells secreting IFN-γ as well as Th2 cells producing IL-4 and IL-5, with IgG1 and IgG2a antibody responses. These results suggest that nasal administration of heat-killed L. casei/40k-OMP elicits both Th1 and Th2 cytokines for induction of antigen-specific antibody responses in oral mucosal as well as systemic compartments and that provide further evidence of the potential of heat-killed recombinant L. casei vaccine for induction of protective immune responses against P. gingivalis infection.
著者
-
ABIKO Yoshimitsu
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
-
Abiko Yoshimitsu
Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud
-
Makimura Masaharu
Department Of Comprehensive Clinical Dentistry Research Institute Of Oral Science Nihon University S
-
Tanaka Hiroyuki
Department Of Anesthesia And Clinical Research Institute National Hospital Organization Kyushu Medic
-
Kurita-Ochiai Tomoko
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
-
YAMAMOTO Masafumi
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University
-
Hashizume Tomomi
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
-
Sewaki Tomomitsu
GenoLac BL Corporation
-
Nozaki Chikateru
Applied Research Department, Kikuchi Research Center, The Chemo-Sero-Therapeutic Research Institute
-
Tanaka Hiroyuki
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
関連論文
- Discoidin Domain Receptor-1 Gene Expression of Osteoblasts on Fibronectin Coated Titanium Disks
- Successful Treatment of Primary Cardiac Lymphoma With Atrioventricular Nodal Block
- PJ-762 The Ratio and Lesion Morphology of Recurrent Restenosis after Reimplantation with Sirolimus-eluting Stent for Restenosis after Sirolimus-eluting Stent Implantation(PJ128,Coronary Revascularization, PCI (DES) 5 (IHD),Poster Session (Japanese),The 73
- PJ-478 The Detection of Cancer with Multidetecter Computed Tomography Coronary Angiography(PJ081,CT/MRI (Coronary/Vascular) 3 (I),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PJ-408 Sirolimus-eluting Stent Implantation for In-stent Restenosis of Bare Metal Stent or Sirolimus-eluting Stent(PJ069,Coronary Revascularization, PCI (Restenosis/Others) 1 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japane
- PE-379 Late Restenosis of Sirolimus Eluting Stent Implantation in Chronic Total Occlusions(PE064,Coronary Revascularization, PCI (DES) 6 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-280 Unique Distribution of Stent Fracture and Stent Fracture with Restenosis after Sirolimus-Eluting Stent Implantation(PE047,Coronary Revascularization, PCI (DES) 4 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circ
- PE-134 Is Previous Angiography as Follow up Useful to Predict Very Late Stent Thrombosis?(PE023,Coronary Revascularization, PCI (DES) 3 (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OJ-255 Usefulness of Stress Myocardial Perfusion Imaging (MPI) in Detecting Significant Coronary Artery Stenosis in Multidetector Row Computed Tomography (MDCT) Era(OJ43,Nuclear Cardiology (Coronary, Myocardium) (I),Oral Presentation (Japanese),The 73rd A
- OJ-121 Predictors of Recurrent Restenosis after Treatment of Bifurcation Lesions with the Two-stent Strategy(OJ21,Coronary Revascularization, PCI (Complex Lesions) 2 (IHD),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese Cir
- OE-403 Stent Fracture and Stent Thrombosis after Sirolimus-eluting Stent Implantation(OE68,Coronary Revascularization, PCI (Restenosis/Others) 4 (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OE-188 Prevalence and Predictors of Early and Late Restenosis after Sirolimus-Eluting Stent Implantation for de novo Lesion(OE32,Coronary Revascularization, PCI (Restenosis/Others) 2 (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of
- PJ-657 Optimal Strategy of Restenosis with Stent Fracture after Sirolimus-eluting stent Implantation(Coronary revascularization, PCI(24)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-258 The 2-year Follow-up Results of Drug Eluting Stent Implantation for the de novo Unprotected Left Main Coronary Artery Disease.(Coronary revascularization, PCI(19)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circ
- PE-049 The Long-Term Predictors of Restenosis and Target-Lesion-Revascularization after Drug-Eluting-Stent Implantation for the de novo Unprotected Left Main Coronary Artery Disease.(Coronary revascularization, PCI(07)(IHD),Poster Session(English),The 72n
- OE-004 Late Restenosis After Sirolimus-eluting Stent Implantation : The Impact of Lesion Complexity(Coronary revascularization, PCI(01)(IHD),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-025 Middle-term angiographic follow-up of BioMatrix^ stent implantation for left main to left anterior descending artery(Forefront Updates in PCI(01)(IHD),Featured Research Session,The 72nd Annual Scientific Meeting of the Japanese Circulation Soc
- PJ-744 Long-term Outcome of Treatment of De Novo Right Coronary Artery Ostial Lesions, Comparison with Drug-eluting Stents and Bare Metal Stents(Coronary revascularization, PCI-20, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-601 Prevalence of Side Effects of Clopidgrel Which was Prescribed to the Patients who Discontinued Ticlopidine due to its Side Effects(Coronary revascularization, PCI-16, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-266 The Angiographical Findings of the Late Restenosis : Comparison Between SES AND BMS Lesions(Restenosis basic/clinical-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Development of an Enzyme Linked Immunosorbent Assay for Direct Determination of Anticancer Drug Vitamin K3 in Serum
- Improved Stability of Metal-Insulator-Diamond Semiconductor Interface by Employing BaF_2 Insulator Film
- THE STUDY OF HYPERTROPHIED RAT HEART : 1. THE NEW METHOD TO MAKE HYPERTROPHIED RAT HEART : Hemodynamics, Cardiac Function (IV) : IV : 48 Annual Scientific Meeting, Japanese Circulation Society
- DHPLCを用いた日本人骨形成不全症(OI)におけるI型コラーゲンの遺伝子解析
- Lactobacillus Acidophilus Strain L-92 Regulates the Production of Th1 Cytokine as well as Th2 Cytokines
- Production of Matrix Metalloproteinases in Human Cultured Mast Cells : Involvement of Protein Kinase C-Mitogen Activated Protein Kinase Kinase-Extracellular Signal-regulated Kinase Pathway
- Establishment of Allergic Dermatitis in NC/Nga Mice as a Model for Severe Atopic Dermatitis(Pharmacology)
- The Effect of a Newly Synthesized Indazole Compound, TAS-3-124, on Experimental Autoimmune Disease(Pharmacology)
- Role of T cells in IgE-dependent triphasic cutaneous reaction caused by dinitrofluorobenzene in the mouse ear : Participation of CD8^+ T cells
- P-53 Effects of Vaticanol (B, C and G), α-Viniferin and Hopeaphenol on the Release of Mediators from Bone Marrow-Derived Mast Cells
- OJ-080 Middle Term Clinical and Angiographic Outcomes of BioMatrix^ Stent Implantation for Left Main Coronary Artery Stenosis(Coronary revascularization, PCI-05, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-243 Predictors of Angiographic Stent Fracture and Stent Fracture with Restenosis after Sirolimus-eluting stent (SES) Implantation(Coronary revascularization, PCI-03, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Effect of Antiallergic Drugs on Interleukin 5-Induced Eosinophil Infiltration of Rat Airways
- ビスホスホネートで治療された骨形成不全症乳幼児の成長に関する検討
- Impaired pyridinoline cross-link formation in patients with osteogenesis imperfecta
- Cell-adhesive Protein Immobilization Using Tresyl Chloride-activation Technique for the Enhancement of Initial Cell Attachment
- Frontier Molecular Orbital Study for the Reaction of Tresyl Chloride
- Effects of Salmeterol Xinafoate and Fluticasone Propionate on Immunological Activation of Human Cultured Mast Cells
- FGFR3-related dwarfism and cell signaling
- Efficacy of growth hormone therapy for patients with skeletal dysplasia
- Connexin 43 expression at an early stage in dog mandibles by β-TCP
- Proteomic Analysis of Apoptosis Induced by Xanthoangelol, a Major Constituent of Angelica keiskei, in Neuroblastoma(Pharmacology)
- Effect of oxygen radical on apoptosis in human submandibular gland cell lines
- T Helper Cytokine Responses Elicited by Nasal Immunization with Heat-Killed Recombinant Lactobacillus casei Expressing Outer Membrane Protein of Porphyromonas gingivalis
- Immunogenicity and Protective Efficacy of Heat-Killed Recombinant Lactobacillus casei Expressing Outer Membrane Protein of Porphyromonas gingivalis
- Qualitative study of the New Bone formation Surrounding the Ti-implant by FTIR and Polarizing Microscope
- Large-scale preparative disk gel electrophoresis with electroosmotic medium pump system
- Comparison of Otoraplin Gene Expressions in Osteoblasts Grown on Fibronectin and GRGDSP Peptide-Coated Titanium Disks
- Identification of Reduced Fibronectin 1 Gene Expression in Osteoblasts following Hydrogen Peroxide Treatment using Subtractive Gene Cloning
- Functional Analysis of the Thioredoxin Domain in Porphyromonas gingivalis HBP35
- Construction of a Chimeric Shuttle Plasmid via a Heterodimer System : Secretion of an scFv Protein from Bacillus brevis Cells Capable of Inhibiting Hemagglutination
- Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide
- Expression of Platelet-Derived Growth Factor Receptor Beta in Dog Mandible by Beta-Tricalcium Phosphate
- Type I Collagen Grafting on Titanium Surfaces Using Low-temperature Glow Discharge
- Purification and Characterization of an Oligo-isomaltosaccharide Synthase from a Streptococcus sobrinus Glucosyltransferase-I Deficient Mutant
- 6. Low-energy laser irradiation stimulates bone formatioun via IGF-I expression in fetal rat calvarial cells.
- IL-1β production and gene expression by peptidoglycan from Lactobacillus casei in human dental pulp cells of deciduous teeth and permanent teeth
- Determination of Short Peptide in a Porphyromonas gingivalis Protein Antigen Recognized by Sera from Periodontitis Patients
- The relationship between premature ageing and immune responses in the oral cavity of Down syndrome
- A Fusion Protein of the Hemagglutinin A Antigenic Region of Porphyromonas gingivalis and Maltose-Binding Protein Induces Antibody Responses in the Oral Cavity
- Oxidative stress induces phosphorylation of the ABC transporter, ATP-binding protein, in Porphyromonas gingivalis
- Gene Cloning and Characterization of Short Immunodominant Hemagglutinin A of Porphyromonas gingivalis
- Reduction of Early Growth Response-1 Gene Expression in Osteoblasts by Hydrogen Peroxide
- A Newly Designed Resonance Frequency Analysis Device for Dental Implant Stability Detection
- Gene Expression of MC3T3-E1 Cells on Fibronectin-immobilized Titanium Using Tresyl Chloride Activation Technique
- Transcutaneous Immunization with P. gingivalis Surface Protein Antigen Induces T Helper 2 Responses in Systemic Tissues
- Nasal Immunization with P. gingivalis Surface Protein Antigen and Cholera Toxin Adjuvant Induces T Helper 2 Responses in Both Mucosal and Systemic Compartments
- Aggregatibacter actinomycetemcomitans Leads to Endothelial Apoptosis and Atherosclerosis Development in Spontaneously Hyperlipidemic Mice
- Functional Analysis of the Thioredoxin Domain in Porphyromonas gingivalis HBP35
- Identification of Inhibin B Gene Expression in Osteoblast by Low Level Laser Irradiation
- Identification of the functional domain in a coaggregation factor from Porphyromonas gingivalis
- Connexin 43 expression at an early stage in dog mandibles by β-TCP
- Analysis of Correlation between Caries Risk Factors to Establish an Effective Clinical Testing and Diagnostic System for Caries Risk
- Reduction of Bone Nodule Formation in MC3T3-E1 Cells by Treatment with Hydrogen Peroxide
- Promoter and Terminator Analysis of 40-kDa Outer Membrane Gene (hbp35) in Porphyromonas gingivalis 381
- Effect of TNF-α on uPA and uPA receptor expressions human gingival fibroblasts
- Effect of Low Level Laser Irradiation on CXCR3 Gene Expression in Rheumatoid Arthritis Joint
- Effect of Low-Level Laser Irradiation on CXCL13 Gene Expression in Rheumatoid Arthritis Rat Joints
- Transcutaneous Immunization with the Outer Membrane Protein of P. gingivalis Elicits Long-term Protective Immunity in the Oral Cavity
- Hydrogen Peroxide Reduced Osteomodulin Gene Expression in MC3T3-E1
- Osteomodulin Gene Expression in Beagle Dog Mandible by Beta-Tricalcium Phosphate
- Age-related changes in IGF-1 expression in submandibular glands of senescence-accelerated mice
- Profiling of differentially expressed genes in human gingival epithelial cells and fibroblasts by DNA microarray
- Stimulation of Mesenchymal-epithelial Transition Factor (c-Met) Expression in Candida Albicans-infected Human Gingival Epithelial Cells and Rat Gingival Epithelium
- Fibroblast growth factor 2 and forskolin induce mineralization-associated genes in two kinds of osteoblast-like cells
- Nasal Vaccination with a Fusion Protein of Hemagglutinin A Antigenic Region and Maltose-Binding Protein Elicits CD4+ T Cell Responses via Toll-Like Receptor 4
- High-Temperature Requirement Protein A1 (HtrA1) Gene Expression in Dog Mandible Bone by β-TCP
- Gene Expression Profiling during Growth in vitro Using a Custom-made Porphyromonas gingivalis Gene Array
- Evaluation of Buffering Capacity of Resting Saliva and Caries Prevalence for Caries Risk Assessment
- Associations among distributions of dental postgraduate residents, dentists and clinical training facilities in Japan
- Differentiation of the human mesenchymal stem cells derived from bone marrow and enhancement of cell attachment by fibronectin
- Analysis of mRNA Expression in Epithelial Layers of Oral Lichen Planus by Laser Microdissection with RT-PCR
- Monitoring of dnaK gene expression in Porphyromonas gingivalis by oxygen stress using DNA microarray
- Mandibular B-Cell Lymphoma in a 3-Year-Old Child: A Clinical Rarity
- Attachment Activity of RGD-Motif Peptides to Osteoblast-Like Cell Line MC3T3-E1
- Osteomodulin Gene Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells into Osteoblastic Differentiation
- Signal Peptide Analyses and Localization of 40-kDa Outer Membrane Protein in Porphyromonas gingivalis 381
- Inhibition of Porphyromonas gingivalis hemagglutinating activity by IgY against a truncated HagA
- Retraction : Promoter and Terminator Analysis of 40-kDa Outer Membrane Gene (hbp35) in Porphyromonas gingivalis 381
- Multi-observer Assessment of Trainee Dentists