Development of an Enzyme Linked Immunosorbent Assay for Direct Determination of Anticancer Drug Vitamin K3 in Serum

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概要

• 論文の詳細を見る

• Synthetic vitamin K3 (VK3, 2-methyl-1,4-naphthoquinone, or menadione) has been shown to have anticancer activity in various human cancer cells. We developed an enzyme-linked immunosorbant assay (ELISA) for determination of VK3 in fetal calf serum (FCS) without any pretreatment. The monoclonal antibody against VK3, which was secreted from an established hybridoma cell line (3A3) has been proven to have highly specific binding to VK3 in cross-reactivity analysis. The full measuring range of the assay extends from 0.39 to 50 μg/ml of VK3. Based on validation analysis, this immunological analysis is a precise, accurate, and sensitive method for determination of VK3. This simple immunological method would deserve to be applicable clinically.

• 2008-08-01

著者

• Tanaka Hiroyuki

  Department Of Cardiology Kurashiki Central Hospital

• Sakamoto Seiichi

  Department of Medicinal Plants Breeding and Pharmacognosy, Graduate School of Pharmaceutical Science

• Sakoda Junichi

  Department of Medicinal Plants Breeding and Pharmacognosy, Graduate School of Pharmaceutical Science

• Morinaga Osamu

  Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University

• Waraporn Putalun

  Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Khon Kaen University

• Tsuchihashi Ryota

  Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Fukuoka University

• Morimoto Satoshi

  Department of Medicinal Plants Breeding and Pharmacognosy, Graduate School of Pharmaceutical Science

• Kinjo Junei

  Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Fukuoka University

• 金城 順英

  熊本大学 医薬

• Sakamoto Seiichi

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Morinaga Osamu

  Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Nagasaki International University

• Morinaga Osamu

  九州大学 薬学府薬用植物育種学

• Morinaga Osamu

  Department Of Internal Medicine Aiseikai Yamashina Hospital

• TSUCHIHASHI Ryota

  Faculty of Pharmaceutical Sciences, Fukuoka University

• Kinjo Junei

  東海大学 農学部

• Kinjo Junei

  Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Fukuoka University

• Kinjo Junei

  福岡大学 薬学部

• Kinjo Junei

  Faculty Of Pharmaceutical Sciences Kumamoto University

• Kinjo Junei

  熊本大学 薬

• Tsuchihashi Ryota

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Tanaka Hiroyuki

  Graduate School of Pharmaceutical Sciences, Kyushu University

• Morimoto Satoshi

  Graduate School of Pharmaceutical Sciences, Kyushu University

• 金城 順英

  熊本大学 薬

• Jung K

  Laboratory Of Immunomodulator Korea Research Institute Of Bioscience And Biotechnology

• KIM Jin-Cheol

  Korea Research Institute of Chemical Technology

• Kinjo J

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Kim J-h

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Jung K

  Korea Res. Inst. Biosci. And Biotechnol. Taejon Kor

• PUTALUN Waraporn

  Faculty of Pharmaceutical Sciences, Khon Kaen University

• Tanaka H

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Sakoda Junichi

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Morimoto Satoshi

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Kinjyo Junei

  Faculty Of Pharmaceutical Sciences Kumamoto University

• Tanaka Hiroyuki

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Putalun Waraporn

  Faculty Of Pharmaceutical Sciences Khon Kaen University

• Tsukikawa Hiroshi

  Department Of Pharmacognosy Graduate School Of Medical Science

• Tanaka H

  Department Of Medicinal Plants Breeding And Pharmacognosy Graduate School Of Pharmaceutical Sciences

• Tanaka Hiroyuki

  Department Of Anesthesia And Clinical Research Institute National Hospital Organization Kyushu Medic

• Morimoto Satoshi

  Department Of Internal Medicine Murakami Memorial Hospital Asahi University

• Morimoto Satoshi

  Department of Endocrinology and Hypertension, Tokyo Womens Medical University, Japan

• MORIMOTO Satoshi

  Department of Endocrinology and Hypertension, Tokyo Women's Medical University

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