Qualitative study of the New Bone formation Surrounding the Ti-implant by FTIR and Polarizing Microscope

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概要

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• Recently, dental implants have gained much attention for a great number of researchers in dental application. Until now, there are few reports about the properties and metabolism related to the bite force response of the newly bone formed surrounding the implants, which is poorly understood. For that reason, the purpose of this study was to elucidate the new bone formation surrounding the implants and its process of maturation by Micro-CT, polarizing microscope and FTIR analysis. Thus, the implants were inserted in the tibia of rabbits. After 4 and 8 weeks of surgical procedure, three-dimensional image of the implant and bone of non-decalcified samples was observed by Micro-CT analysis; bone structure and its maturity were seen by polarizing microscope; and finally, the PO_4^<3->, CCO_3^<2-> and Amide I elements included in the interface bone-implant and in the new bone during the course time were examined by FTIR analysis. The results of this study, analyzed by this 3 methods, demonstrated that: 1) It was observed a qualitative difference in the new bone formation and the existing bone at 4 and 8 weeks of implantation; 2) There was also a qualitative difference in the newly bone formed at 4 and 8 weeks; 3) The maturation of the new bone started from the exhibiting bone; and 4) None of the methods used in current study destroyed the samples, and reproducibility results were obtained. In conclusion, we suggest a possible qualitative evaluation of new bone formation surroundings the Ti implant.

• 硬組織再生生物学会の論文

著者

• Nakada Hiroshi

  Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University

• LEGEROS Racquel

  ニューヨーク大学

• Nakada Hiroshi

  Department Of Gnatho-oral Prosthetic Rehabilitation Nihon University School Of Dentistry At Matsudo

• Makimura Masaharu

  Department of Comprehensive Clinical Dentistry, Research Institute of Oral Science, Nihon University

• Sakae Toshiro

  Department of Anatomy, Nihon University School of Dentistry at Matsudo

• Sakae Toshiro

  日本大学松戸歯学部 組織発生解剖学

• Sakai Takeshi

  Inst. Of Mineralogy Petrology And Economic Geology Graduate School Of Sci. Tohoku Univ.

• Sakae Toshiro

  Department Of Anatomy Nihon University School Of Dentistry At Matsudo

• Legeros Racquel

  Department Of Biomaterials And Biomimetics New York University College Of Dentistry

• Legeros Racquel

  Department Of Biomaterials And Biomimetics College Of Dentistry New York University

• Legeros Racquel

  日本大学松戸歯学部 組織・発生・解剖学講座

• Nakada Hiroshi

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• Sakae Toshiro

  Department Of Anatomy Ii Nihon University School Of Dentistry At Matsudo

• Numata Yasuko

  Nihon University Graduate School Of Dentistry At Matsudo Complete Denture Prosthodontics

• Kobayashi Kihei

  Nihon University Research Center

• Tanaka Shigeo

  Department Of Anatomy Nihon University School Of Dentistry At Matsudo

• Sakai Takeshi

  Nihon Univ. Tokyo Jpn

• Kimura-Suda Hiromi

  PerkinElmer Japan Co., Ltd

• Sakae Toshiro

  Departments Of Oral Histology Nihon University School Of Dentistry

• Kimura-suda Hiromi

  Perkinelmer Japan Co. Ltd

• Nakada Hiroshi

  Graduate School Of Science And Technology Nihon University

• Kobayashi Kihei

  Nihon University Of Dentistry At Matsudo

• Makimura Masaharu

  Department Of Comprehensive Clinical Dentistry Research Institute Of Oral Science Nihon University S

• Makimura Masaharu

  Department Of Laboratory Medicine For Dentistry Nihon University School Of Dentistry At Matsudo

• MAKIMURA Masaharu

  Department of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo

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