Nasal Vaccination with a Fusion Protein of Hemagglutinin A Antigenic Region and Maltose-Binding Protein Elicits CD4+ T Cell Responses via Toll-Like Receptor 4
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概要
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Our previous study demonstrated that nasal immunization with the fusion protein of the 25-kDa antigenic hemagglutinin A antigenic region and maltose-binding protein (25k-hagA-MBP) elicited high levels of 25k-hagA-specific antibody responses in both systemic and oral mucosal areas. In this study, we further elucidated the immunogenicity of 25k-hagA-MBP using Toll-like receptor (TLR) 4 gene-deficient (TLR4-/-) mice. When CD4+ T cells isolated from the spleen and cervical lymph nodes (CLN) of 25k-hagA-MBP-stimulated TLR4+/+ mice were restimulated with 25k-hagA in vitro, significant proliferative responses were induced. In contrast, only low levels of CD4+ T cell proliferation were induced in restimulated cells isolated from TLR4-/- mice. Analysis of cytokine responses showed that nasal administration of 25k-hagA-MBP to TLR4+/+ mice produced significant levels of IL-6 and IL-10 in cells from the spleen and CLN. However, these cytokine responses were marginal in TLR4-/- mice nasally immunized with 25k-hagA-MBP. These results suggest that nasal immunization with 25k-hagA-MBP induces 25k-hagA-specific CD4+ T cells via a TLR4-dependent pathway.
著者
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Ikeda Takahiro
Department Of Applied Physics Faculty Of Engineering Hokkaido University
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Abiko Yoshimitsu
Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud
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Kurita-Ochiai Tomoko
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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YAMAMOTO Masafumi
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University
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Hashizume Tomomi
Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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Du Yuan
Departments of Oral Immunology, Nihon University School of Dentistry at Matsudo
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Tsurumi Atsushi
Departments of Oral Immunology, Nihon University School of Dentistry at Matsudo
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Tomita Naoya
Departments of Oral Immunology, Nihon University School of Dentistry at Matsudo
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Du Yuan
Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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Hashizume Tomomi
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo
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