Effect of TNF-α on uPA and uPA receptor expressions human gingival fibroblasts

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概要

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• Tumor necrotizing factor-alpha (TNF-α) is thought to be an important inflammatory cytokine in the progression of periodontitis. Plasminogen activator (PA)/plasmin cascade plays an important role in the inflammatory process through the activation of metalloprotease. The binding of urokinase-type PA (uPA) to its receptor (uPAR) has been proposed as an important feature of cellular processes requiring extracellular matrix degradation. The uPAR attaches on the cell surface with a glycosylphophatidylinositol (GPI) anchor and serves to localize and accelerate the proteolysis cascade. However, the relation of TNF-α and (PA)/plasmin cascade activation by uPAR in periodontitis is not clear. In this study, we primary-cultured human-gingival-tissue-derived fibroblasts (hGF) and examined the effect of TNF-α on uPA and uPAR expression in hGF. PA activities in the conditioned medium and cell lysates of hGF were increased by TNF-α treatment. In addition, PA activity released by phosphatidylinositol-specific phospholipase C, which detaches the GPI anchor, was also increased by TNF-α treatment. Furthermore, TNF-α increased the mRNA levels of both uPA and uPAR by reverse transcription-polymerase chain reaction analysis. These findings suggest that the enhancement of uPA and uPAR expressions by TNF-α may play an important role in the progression of periodontitis through pericellular proteolysis.

• 日本大学の論文

著者

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Abiko Yoshimitsu

  Department Of Biochemistry And Research Institute Of Oral Science Nihon University School Of Dentist

• Mizuguchi Toshiyuki

  Department of Biochemistry, Nihon University School of Dentistry at Matsudo

• Mizuguchi Toshiyuki

  Department Of Biochemistry Nihon University School Of Dentistry At Matsudo

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