Nasal Immunization with P. gingivalis Surface Protein Antigen and Cholera Toxin Adjuvant Induces T Helper 2 Responses in Both Mucosal and Systemic Compartments

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概要

• 論文の詳細を見る

• It is well established that Porphyromonas gingivalis is one of the major pathogens of adult periodontitis. P. gingivalis produces an outer membrane protein with a molecular mass of 40-kDa (40k-OMP). In the present study, in order to assess the potential for application of 40k-OMP in the development of an anti-periodontal disease vaccine, we analyzed 40k-OMP-specific CD4^+ T helper (Th) cell responses induced in the mucosal as well as systemic compartments when 40k-OMP was administered nasally. When CD4^+ T cells that were isolated from cervical lymph nodes (CLN) and spleens of mice immunized with 40k-OMP plus cholera toxin (CT) as adjuvant were re-stimulated with 40k-OMP in vitro, significant levels of proliferative responses were induced. In contrast, essentially no increased proliferation occurred in CLN and spleens taken from mice given 40k-OMP alone. Analysis of T helper (Th) 1 (IFN-γ) and Th2 (IL-4, IL-5 and IL-6) cytokine responses showed that 40k-OMP-specific Th cells from both CLN and the spleen produced significant levels of IL-4, IL-5 and IL-6 but did not result in changes in IFN-γ production. In contrast, marginal levels of IL-4, IL-5 and IL-6 production were seen in mice given 40k-OMP alone nasally. These results suggest that nasal administration of 40k-OMP plus CT as an adjuvant can elicit 40k-OMP-specific Th2-type cytokine responses in both mucosal and systemic compartments. Further, the nasal 40k-OMP vaccine possesses the potential for antiperiodontal vaccine.

• 日本大学の論文

著者

• ABIKO Yoshimitsu

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Yamamoto Masafumi

  Department of Oral Medicine, Research Institute of Oral Science, Nihon University School of Dentistr

• Yamamoto M

  Ntt Electronics Corporation

• Yamamoto M

  Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology:department Of Oral Medic

• Abiko Yoshimitsu

  Departments Of Biochemistry Research Institute Of Oral Science Nihon University School Of Dentistry

• HAYAKAWA Mitsuo

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Maeba Satomi

  Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology

• Hayakawa Mitsuo

  Departments Of Chemistry Research Institute Of Oral Science Nihon University School Of Dentistry At

• Yamamoto Masafumi

  Department Of Materials Science Faculty Of Engineering Chiba University

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Yamamoto M

  Nihon Univ. School Of Dentistry At Matsudo Chiba Jpn

• Maeba Satomi

  Clinical Pathology, Nihon University Graduate School of Dentistry at Matsudo

• Namikoshi Jun

  Clinical Pathology, Nihon University Graduate School of Dentistry at Matsudo

• Namikoshi Jun

  Department of Oral Medicine, Nihon University School of Dentistry at Matsudo

• Otake Shigeo

  Department of Oral Medicine, Research Institute of Oral Science Nihon University School of Dentistry

• Yuda M

  Yonago National Coll. Technol. Tottori

• Otake Shigeo

  Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology:department Of Oral Medic

• Namikoshi Jun

  Department Of Oral Medicine Nihon University School Of Dentistry At Matsudo

• Yamamoto Masafumi

  Department Of Computer Science And Engineering Nagoya Institute Of Technology

• YAMAMOTO Masafumi

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University

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