Immunogenicity and Protective Efficacy of Heat-Killed Recombinant Lactobacillus casei Expressing Outer Membrane Protein of Porphyromonas gingivalis

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概要

• 論文の詳細を見る

• This study seeks to access the potential of a heat-killed recombinant Lactobacillus casei expressing 40-kDa outer membrane protein of Porphyromonas gingivalis (L. casei/40k-OMP) as a nasal vaccine against P. gingivalis infection. The 40-kDa outer membrane protein of P. gingivalis (40k-OMP) were expressed on the surface of L. casei using poly-γ-glutamate symthetase A (pgsA). Nasal immunization with heat-killed L. casei/40k-OMP induced significant 40k-OMP-specific serum IgG, IgA and saliva IgA antibody responses. Antibody-forming cell analysis revealed high numbers of 40k-OMP-specific IgA antibody-forming cells in the submandibular glands of mice given heat-killed L. casei/40k-OMP. Importantly, nasal administration of heat-killed L. casei/40k-OMP resulted in the significant reduction of alveolar bone loss caused by oral infection with P. gingivalis. Because L. casei is one of major probiotic bacterias, nasal administration of heat-killed L. casei/40k-OMP should be an effective and safe mucosal vaccine for humans against P. gingivalis infection and may be an important tool for prevention of chronic periodontitis.

著者

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Makimura Masaharu

  Department Of Comprehensive Clinical Dentistry Research Institute Of Oral Science Nihon University S

• Tanaka Hiroyuki

  Department Of Anesthesia And Clinical Research Institute National Hospital Organization Kyushu Medic

• Kurita-Ochiai Tomoko

  Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo

• YAMAMOTO Masafumi

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University

• Hashizume Tomomi

  Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo

• Sewaki Tomomitsu

  GenoLac BL Corporation

• Nozaki Chikateru

  Applied Research Department, Kikuchi Research Center, The Chemo-Sero-Therapeutic Research Institute

• Kurita-Ochiai Tomoko

  Departments of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo

• Makimura Masaharu

  Departments of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo

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