Profiling of differentially expressed genes in human gingival epithelial cells and fibroblasts by DNA microarray
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概要
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Gingival epithelial cells and fibroblasts play important roles and have a harmonious relationship under normal and disease conditions, but the precise differences between theses cells remain unknown. To study the differences in gene expression between human gingival epithelial cells (HGE) and human gingival fibroblasts (HGF), mRNA was recovered from primary cultured cells and analyzed using cDNA microarray technology. The cDNA retrotranscribed from equal quantities of mRNA was labeled with the fluorescent dyes Cy5 and Cy3. The mixed probes were then hybridized with 7276 genes on the DNA microarray, after which fluorescence signals were scanned and further analyzed using GeneSpring software. Of the 7276 genes screened, 469 showed expression levels that were more than 2-fold greater in HGE than in HGF, while 293 showed expression levels that were more than 2-fold greater in HGF than in HGE. To confirm the reliability of the microarray results, keratin K5 and desmocolin, and vimentin and gp130, which showed higher mRNA levels in HGE and HGF, respectively, were selected and their mRNA levels were further analyzed by RT-PCR. The results of RT-PCR correlated well with those of microarray analysis. The present findings using a DNA microarray to detect differences in the gene expression profiles of HGE and HGF may be beneficial for genetic diagnosis of periodontal tissue metabolism and periodontal diseases. (J. Oral Sci. 46, 19-24, 2004)
著者
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OHTA Mitsuhiro
Department of Applied Chemistry, Muroran Institute of Technology
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Abiko Yoshimitsu
Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud
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Hiratsuka Koichi
Department Of Surgery I Oita Medical University
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Kiyama-kishikawa Michiko
Department Of Biochemistry And Research Institute Of Oral Science Nihon University School Of Dentist
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Tsushima Katsumasa
Department of Biochemistry, Nihon University School of Dentistry at Matsudo
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Sasahara Hiroshige
Department of Oral Diagnosis, Nihon University School of Dentistry at Matsudo
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Hiratsuka Koichi
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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