Transcutaneous Immunization with P. gingivalis Surface Protein Antigen Induces T Helper 2 Responses in Systemic Tissues

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概要

• 論文の詳細を見る

• Porphyromonus gingivalis is a major pathogen of chronic periodontitis. An outer membrane protein with a molecular mass of 40-kDa (40k-OMP) is a highly immunogenic surface protein produced by P. gingivalis. In this study, to develop an effective vaccine against P. gingivalis infection, we assessed T helper (Th) cell responses in systemic and mucosal compartments after 40k-OMP was administered trans-cutaneously. When CD4^+ T cells isolated from the spleens of mice immunized with 40k-OMP alone or 40k-OMP plus cholera toxin were restimulated with 40k-OMP in vitro, significant levels of proliferative responses were induced. In contrast, only low levels of CD4^+ T cell proliferation were induced in cervical lymph nodes. Analysis of Th1 [interferon (IFN)-γ] and Th2 [interleukin (IL)-4, IL-5, and IL-6] cytokine responses showed that 40k-OMP-specific Th cells from the spleen produced significant levels of IL-4, IL-5, and IL-6 but did not trigger changes in IFN-γ production. These results suggest that transcutaneous administration of 40k-OMP can elicit 40k-OMP-specific Th2-type cytokine responses in systemic, but not mucosal, lymphoid tissues.

• 日本大学の論文

著者

• ABIKO Yoshimitsu

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Yamamoto M

  Ntt Electronics Corporation

• Yamamoto M

  Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology:department Of Oral Medic

• Abiko Yoshimitsu

  Departments Of Biochemistry Research Institute Of Oral Science Nihon University School Of Dentistry

• HAYAKAWA Mitsuo

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Maeba Satomi

  Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology

• Hayakawa Mitsuo

  Departments Of Chemistry Research Institute Of Oral Science Nihon University School Of Dentistry At

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Yamamoto M

  Nihon Univ. School Of Dentistry At Matsudo Chiba Jpn

• Maeba Satomi

  Clinical Pathology, Nihon University Graduate School of Dentistry at Matsudo

• Otake Shigeo

  Clinical Pathology, Nihon University Graduate School of Dentistry at Matsudo

• Namikoshi Jun

  Clinical Pathology, Nihon University Graduate School of Dentistry at Matsudo

• Yamamoto Masafumi

  Clinical Pathology, Nihon University Graduate School of Dentistry at Matsudo

• Namikoshi Jun

  Department of Oral Medicine, Nihon University School of Dentistry at Matsudo

• Yuda M

  Yonago National Coll. Technol. Tottori

• Otake Shigeo

  Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology:department Of Oral Medic

• Namikoshi Jun

  Department Of Oral Medicine Nihon University School Of Dentistry At Matsudo

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