Transcutaneous Immunization Induces Antigen-Specific Antibody Responses in the Oral Cavity
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概要
- 論文の詳細を見る
Transcutaneous immunization is a new method of vaccination that utilizes a topical application of antigen to intact skin for induction of immune responses. In this study, we have assessed the potential for the application of transcutaneous immunization for the development of a novel vaccine-delivery system. When BALB/c mice were immunized with ovalbumin (OVA) by direct application to shaved skin, OVA-specific serum IgG antibody responses were induced; however, none was induced in saliva. On the other hand, when OVA was given with cholera toxin (CT) as adjuvant, higher levels of OVA-specific serum IgG antibody responses were induced than that of OVA alone. Furthermore, OVA-specific IgG antibody responses were detected in the saliva of mice immunized with OVA plus CT. Antibody-forming cell (AFC) analysis confirmed the antibody titers by revealing significant numbers of OVA-specific IgG AFCs in the spleen and salivary gland. In addition, mononuclear cells from the spleen and cervical lymph nodes of mice immunized with OVA plus CT exhibited significant levels of proliferative responses when restimulated with OVA in vitro. These results indicate that transcutaneous immunization may be a effective vaccine delivery system for the induction of protein antigen-specific antibody responses in the oral cavity.
- 日本大学の論文
著者
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Yamamoto M
Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology:department Of Oral Medic
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Maeba Satomi
Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology
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Yamamoto Masafumi
Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology:department Of Oral Medic
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Maeda Satomi
Nihon University Graduate School of Dentistry at Matsudo, Clinical Pathology
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Namikoshi Jun
Department of Oral Medicine, Nihon University School of Dentistry at Matsudo
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Indo Jiro
Department of Chemistry, Research Institute of Oral Science, Nihon University School of Dentistry at
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Takahashi Mitsuji
Department of Oral Medicine, Nihon University School of Dentistry at Matsudo
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Otake Shigeo
Nihon University Graduate School of Dentistry at Matsudo, Clinical Pathology
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Yuda M
Yonago National Coll. Technol. Tottori
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Takahashi Mitsuji
Department Of Oral Medicine Nihon University School Of Dentistry At Matsudo
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Indo Jiro
Department Of Chemistry And Research Institute Of Oral Science Nihon University School Of Dentistry
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Indo Jiro
Department Of Chemistry Research Institute Of Oral Science Nihon University School Of Dentistry At M
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Otake Shigeo
Nihon University Graduate School Of Dentistry At Matsudo Clinical Pathology:department Of Oral Medic
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Namikoshi Jun
Department Of Oral Medicine Nihon University School Of Dentistry At Matsudo
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