Identification of peptide motif that binds to the surface of zirconia
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概要
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A zirconia-binding peptide motif was identified using a peptide phage display system. Yttria stabilized zirconia beads and discs were used as the target. Quartz crystal microbalance was used to monitor the binding of phages to zirconia. Starting from a library of phages displaying random sequences of 12-mer peptides, we repeated cycles of biopanning against zirconia beads. After four cycles of biopanning, we isolated a phage clone Φ#17. DNA sequencing of the corresponding portion of Φ#17 unexpectedly revealed that it displayed a 58-mer peptide (amino acid sequence: WMPSDVDINDPQGGGSRPNLHQPKPAAEAASKKKSENRKVPFYSHSWY-SSMSEDKRGW). We found that Φ#17 had a 300-fold, significantly higher binding affinity for zirconia discs than phages displaying no peptide. In quartz crystal microbalance assay, a rapid increase in energy dissipation was observed from Φ#17 but not from the control phages, indicating that Φ#17 binds to the surface of zirconia via its displayed peptide. We successfully identified a peptide motif that binds zirconia.
- 日本歯科理工学会の論文
- 2011-11-01
著者
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Sato Masaaki
Department Of Dental Science Institute Of Personalized Medical Science Health Sciences University Of
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INOUE Takashi
Oral Health Science Center, Tokyo Dental College
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NASHIMOTO MASANORI
Department of Pathology, Tokyo Dental College
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Inoue T
Department Of Pathophysiology Tokyo Dental College
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Shimono Masaki
Pathology Tokyo Dental College
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Shiba Kiyotaka
Division Of Protein Engineering Cancer Institute Japanese Foundation For Cancer Research
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HASHIMOTO Kazuhiko
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
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INOUE TAKASHI
Division of Diabetes, Digestive, and Kidney Diseases, Department of Clinical Molecular Medicine, Kob
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Yoshinari Masao
Oral Health Science Center Hrc7 Tokyo Dental College:department Of Dental Materials Science Tokyo De
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Yoshinari Masao
Division Of Oral Implants Research Oral Health Science Center Tokyo Dental College
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Matsuzaka Kenichi
Oral Health Science Center Hrc7 Tokyo Dental College:department Of Clinical Pathophysiology Tokyo De
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Hashimoto Kazuhiko
Division Of Oral Implants Research Oral Health Science Center Tokyo Dental College
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MATSUZAKA Kenichi
Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College
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Yoshinari Masao
Division Of Oral Implants Research Oral Health Science Center Tokyo Dental Collage
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Inoue Takashi
Division Of Diabetes Digestive And Kidney Diseases Department Of Clinical Molecular Medicine Kobe University Graduate School Of Medicine
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SHIBA Kiyotaka
Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research
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