The effect of surface pore size on the differentiation of rat bone marrow cells: morphological observations and expression of bone related protein mRNA
スポンサーリンク
概要
- 論文の詳細を見る
The purpose of this study was to investigate the behavior of rat bone marrow cells (RBM) growing on surfaces with different pore sizes. RBM behavior on Millipore filters (MF-Millipore^<TM> membrane filter) made from cellulose mixed esters with 5 different pore surfaces (0.45μm, 1.2μm, 3.0μm, 5.0μm and 8.0μm) were compared in terms of morphological changes on the different pore sizes. Furthermore, the expressions of osteopontin and osteocalcin mRNAs were investigated. On the 1.2μm and 3.0μm pore surfaces, RBM attached to the substrate well, but cells on the 5.0μm and 8.0μm pore surfaces invaded deeply into the pores. Higher levels of both osteopontin and osteocalcin mRNA expression were always observed in cells cultured on the 1.2μm filter. These results suggest that the 1.2μm Millipore filter pore size is the most suitable for inducing RBM to differentiate into an osteoblastic phenotype among these surfaces and is probably related to production of the ECM but not to the phenomenon of cell spreading.
- 東京歯科大学の論文
著者
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Sato Masaaki
Department Of Dental Science Institute Of Personalized Medical Science Health Sciences University Of
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Saitoh M
Department Of Dental Science Institute Of Personalized Medical Science Health Sciences University Of
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SHIMONO Masaki
Oral Health Science Center HRC7, Tokyo Dental College
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INOUE Takashi
Oral Health Science Center, Tokyo Dental College
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NASHIMOTO MASANORI
Department of Pathology, Tokyo Dental College
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MATSUZAKA KENICHI
Oral Health Science Center, Department of Clinical Pathophysiology, Tokyo Dental College
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YOSHINARI MASAO
Oral Health Science Center, Department of Dental Material Science, Tokyo Dental College
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Saitoh Masato
Department Of Dental Science Institute Of Personalized Medical Science Health Sciences University Of
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Inoue T
Department Of Pathophysiology Tokyo Dental College
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Shimono Masaki
Oral Health Science Center Department Of Pathology Tokyo Dental College
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Shimono M
Oral Health Science Center Hrc7 Tokyo Dental College
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Shimono Masaki
Pathology Tokyo Dental College
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Yoshinari Masao
Oral Health Science Center Hrc7 Tokyo Dental College:department Of Dental Materials Science Tokyo De
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Yoshinari Masao
Department Of Dental Materials Science And Oral Health Science Center Tokyo Dental College
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Matsuzaka Kenichi
Oral Health Science Center Hrc7 Tokyo Dental College:department Of Clinical Pathophysiology Tokyo De
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Yoshinari M
Department Of Dental Materials Science And Oral Health Science Center Tokyo Dental College
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Matsuzaka K
Oral Health Science Center Hrc7 Tokyo Dental College:department Of Clinical Pathophysiology Tokyo De
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Yoshinari M
Oral Health Science Center Hrc7 Tokyo Dental College:department Of Dental Materials Science Tokyo De
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Shintani Masuro
Laboratory Of Brain Research Oral Health Science Center Tokyo Dental College
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Matsuzaka Kenichi
Oral Health Science Center Hrc7 Tokyo Dental College
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Nashimoto Masanori
Department Of Pathology Tokyo Dental College
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Yoshinari M
Oral Health Science Center Tokyo Dental College
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Inoue T
Oral Health Science Center Tokyo Dental College
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Nashimoto M
Department of Pathology, Tokyo Dental College
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