Different Sets of cis-Elements Contribute to the Expression of a Catalase Gene from Castor Bean during Seed Formation and Postembryonic Development in Transgenic Tobacco : GENE STRUCTURE AND EXPRESSION

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概要

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• Deletion analysis of the promoter region of a gene for catalase, cat2, from castor bean (Ricinus communis) was performed to identify the cis-regulatory elements responsible for the expression of a β-glucuronidase (GUS) fusion gene during seed formation and postembryonic development in transgenic tobacco. The analysis showed that multiple cis-elements contribute to the activity of the cat2 promoter during seed formation and postembryonic development. The 5'-upstream regions from -1,241 to -816 bp, from -720 to -682 bp, and from -632 to -535 bp, relative to the site of initiation of translation of cat2, contributed positively to the activity of the cat2 promoter during both stages. By contrast, the region from -816 to -720 bp had a negative effect at both stages. The region from -682 to -632 bp contributed positively to the activity during seed formation but negatively during postembyonic development. Histochemical analysis revealed that the multiple cis-elements determined not only the level of expression of the chimeric gene but also the tissue-specificity of such expression. For example, the region from -1,241 to -816 bp allowed expression of the chimeric gene in the axis of the embryo of the dry seed, as well as in the cortex of the middle part of the hypocotyl and at the base of epicotyl in the young seedling.

• 日本植物生理学会の論文

著者

• NAKAMURA Kenzo

  Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University

• Asahi Tadashi

  Laboratory of Biochemistry, School of Agriculture, Nagoya University

• Nakamura K

  Nagoya Univ. Nagoya Jpn

• Hattori Tsukaho

  Laboratory of Biochemistry, School of Agriculture, Nagoya University

• Hattori Tsukaho

  Center for Molecular Biology and Genetics, Mie University

• Suzuki Masaharu

  Laboratory Of Biochemistry School Of Agriculture Nagoya University

• Hattori Tsukaho

  Center For Molecular Biology And Genetics Mie University:department Of Bioresources School Of Biores

• Hattori Tsukaho

  Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University

• Hattori Tsukaho

  Suntory Institute For Bioorganic Research

• Koide Yasuhiro

  Laboratory of Biochemistry, School of Agriculture, Nagoya University

• Koide Yasuhiro

  Lab. Biochem. Grad. School Of Bioagr. Sci. Nagoya Univ.

• Koide Yasuhiro

  Laboratory Of Biochemistry School Of Agriculture Nagoya University

• Nakamura K

  Lab. Biochem. Grad. School Of Bioagr. Sci. Nagoya Univ.:dept. Developmental Biol. Natl. Inst. Basic

• Asahi T

  Fukui Prefectural Univ. Fukui Jpn

• Asahi Tadashi

  Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University

• Suzuki M

  Division Of Biological Sciences Graduate School Of Science Hokkaido University

• Nakamura Kenzo

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Nakamura Kenzo

  Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University

• Asahi Tadashi

  Laboralory Of Biochemistry Faculty Of Agriculture Nagoya University

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