Modification of OGUR-ROSEN method for estimation of RNA in fresh and wounded sweet potato root tissues and change in RNA contents after the cutting
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概要
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The suitability of the OGUR-ROSEN method for RNA extraction from sweet potato root tissue was investigated. The PCA extract from the freshly sliced tissue was colorless and showed a typical spectrum of RNA. However, as the slices were incubated for 1 to 3 days (wounded tissues), extract from them was brownish and revealed no typical spectrum of RNA. When wounded tissue was treated with acetone containing isoascorbic acid and the resultant acetone powder was treated with PCA, extract indicated the spectrum very close to that of RNA. Interfering substances still remaining were removed satisfactorily with charcoal which adsorbed RNA selectively. Thus, RNA could be assayed as the OD_<260>-difference between PCA extract from the acetone powder and a fraction of the extract non-adsorbable on charcoal. Time course analysis of RNA con-tents during incubation of the tissue slices disclosed a remarkable increase in the total RNA in the initial stage of incubation.
- 日本植物生理学会の論文
著者
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Asahi Tadashi
Laboratory of Biochemistry, School of Agriculture, Nagoya University
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Nawa Yoshihiko
Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University
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Nawa Yoshihiko
Laboratory Of Biochemistry Faculty Of Agriculture Nagoya University
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URITANI IKUZO
Laboratory of Biochemistry, Faculty of Agriculture,Nagoya University
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Uritani Ikuzo
Laboratory Of Biochemistry And Institute For Biochemical Regulation Faculty Of Agriculture Nagoya Un
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Asahi Tadashi
Laboralory Of Biochemistry Faculty Of Agriculture Nagoya University
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