Biosynthesis of furano-terpenes by sweet potato cell culture

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Callus was induced from sweet potato root tissue on an agar medium containing Heller's minerals, vitamins, 2,4-D, yeast extract and sucrose. Furano-terpenes were scarcely detected in the callus. However, when the callus was transferred to a liquid culture medium and incubated with reciprocal shaking, furano-terpenes were rapidly produced mainly in the culture medium. Furano-terpene production by the cell culture was suppressed by addition of Ceratocystis fimbriata spores or HgCl_2 to the culture medium. Yeast extract and sucrose in the culture medium were important for furano-terpene production. 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity increased in the cells, followed by the production of furano-terpenes. The TLC pattern of furano-terpenes produced by the cell culture was essentially the same as that produced by sweet potato root tissue infected by C.fimbriata or treated with HgCl_2, but the quantitative proportion of the individual furano-terpenes in the former differed markedly from that in the latter.