Effects of cycloheximide, actinomycin D and ethylene on the increase and subsequent decrease in acid invertase activity in wounded sweet potato

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In wounded sweet potato root tissue, the mechanisms of the increase and sub-sequcnt decrease of acid invertase activity were examined in compariosn with phe-nylalanine ammonia lyase (PAL) and peroxidase activities. The increase in acid invertase and PAL activities in response to wounding was inhibited by both cyclo-heximide and actinomycin D. However, the increase in peroxidase activity was inhibited by cycloheximide but was not inhibited by actinomycin D, which repressed RNA synthesis 60 to 70%. The increase in acid invertase activity was supressed by ethylene, while PAL and peroxidase activities were stimulated. The results of cycloheximide co-treatment suggest that the fluctuation in enzyme activities by ethylene treatment involves de nove protein synthesis. Further, the effect of ethylene on enzyme activities was under competition by carbon dioxide. The decrease in acid invertase activity at the late stage of incubation was stimulated by cycloheximide treatment, but that of PAI. activty was prevented. The results suggest the existence of different inactivating systems between acid invertase and PAL.
日本植物生理学会の論文