Crystallization and Properties of Amine Dehydrogenase from Pseudomonas sp. (Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
An amine dehydrogenase was purified and crystallized from the cell free extract of a pseudomonas sp., isolated from soil by means of the enrichment technique. The crystalline enzyme gave a single band on polyacrylamide gel electrophoresis and the molocular weight of the enzyme was estimated to be 100,000 by gel filtration on a Sephadex column, Upon SDS-gel electrophoresis, the enzyme was dissociated into two nonidentical subunits having molocular weights of 60,000 (dehydrogenase) and 39,000 (crtochrome c). The absorption spectrum of the enzyme showed absorption maxima at 550nm, 524nm, 411nm, and 280nm, and a broad shoulder at around 350nm, indicating that the enzyme was purified as a dehydrogenase-cytochrome c complex. The prosthestic group of the dehydrogenase was identified as covalently bound pyrroloquinoline quinone. The enzyme showed a broad substrate specificity toward various amines including aliphatic monoamines, aliphatic diamines, aromatic amines and polyamines.
- 社団法人日本農芸化学会の論文
- 1988-09-23
著者
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Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Adachi Osao
Laboratory Of Applied Microbiology Department Of Biological Chemistry Faculty Of Agriculture Yamaguc
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SHINAGAWA Emiko
Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Faculty of Agriculture, Ya
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Ameyama M
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Ameyama Minoru
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Faculty Of Agriculture Yamag
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Matsushita Kazunobu
Laboratory Of Applied Microbiology Department Of Biological Chemistry Faculty Of Agriculture Yamaguc
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NAKASHIMA Koji
Department of Neurosurgery, Fujigaoka Hospital, Showa University
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Nakashima Koji
Department Of Laboratory Medicine St. Luke's College Of Nursing
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Nakashima Koji
Department of Electrical and Electronics Engineering, Aichi Institute of Technology, Yakusa, Toyota, Aichi 470-0392, Japan
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