Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production

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概要

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• NADP-Dependent shikimate dehydrogenae (SKDH, EC 1.1.1.25) was purified from Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.

• 社団法人 日本農芸化学会の論文
• 2006-11-23

著者

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University

• Toyama Hirohide

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• Tanaka Y

  School Of Pharmaceutical Sciences Kitasato University The Kitasato Institute

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Tanaka H

  External Science Affairs Fujisawa Pharmaceutical Co. Ltd.

• Murata Kousaku

  Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O

• Momma Keiko

  Laboratory Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• 松下 一信

  山口大 農

• Adachi O

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Ano Yoshitaka

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Applied Bioscience, Faculty of Agriculture, Ehime University

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