Alterations of cellular physiology in Escherichia coli in response to oxidative phosphorylation impaired by defective F1-ATPase
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概要
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The physiological changes in an F1-ATPase-defective mutant of Escherichia coli W1485 growing in a glucose-limited chemostat included a decreased growth yield (60%) and increased specific rates of both glucose consumption (168%) and respiration (171%). Flux analysis revealed that the mutant showed approximately twice as much flow in glycolysis but only an 18% increase in the tricarboxylic acid (TCA) cycle, owing to the excretion of acetate, where most of the increased glycolytic flux was directed. Genetic and biochemical analyses of the mutant revealed the downregulation of many TCA cycle enzymes, including citrate synthase, and the upregulation of the pyruvate dehydrogenase complex in both transcription and enzyme activities. These changes seemed to contribute to acetate excretion in the mutant. No transcriptional changes were observed in the glycolytic enzymes, despite the enhanced glycolysis. The most significant alterations were found in the respiratory-chain components. The total activity of NADH dehydrogenases (NDHs) and terminal oxidases increased about twofold in the mutant, which accounted for its higher respiration rate. These changes arose primarily from the increased (3.7-fold) enzyme activity of NDH-2 and an increased amount of cytochrome bd in the mutant. Transcriptional upregulation appeared to be involved in these phenomena. As NDH-2 cannot generate an electrochemical gradient of protons and as cytochrome bd is inferior to cytochrome bo3 in this ability, the mutant was able to recycle NADH at a higher rate than the parent and avoid generating an excess proton-motive force. We discuss the physiological benefits of the alterations in the mutant.
- American Society for Microbiologyの論文
著者
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MATSUSHITA Kazunobu
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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冨田 房男
北海道大学農学部
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冨田 房男
Hokkaido Study Center The University Of Air
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横田 篤
北大農
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Yokota Atsushi
Lab. Of Microbial Physiology Res. Fac. Of Agriculture Hokkaido Univ. Kita 9 Nishi 9 Kita-ku Sapporo
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Yokota Atsushi
Laboratory Of Bioorganic Chemistry
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Yokota A
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Yokota Atsushi
Hokkaido Univ. Sapporo Jpn
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Wada Masaru
Hokkaido Univ. Sapporo Jpn
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Tomita Fusao
Hokkaido University:hokkaido Study Center University Of The Air
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Tomita Fusao
Laboratory Of Applied Microbiology
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Tomita Fusao
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Tomita Fusao
Department Of Agricultural Biochemistry Faculty Of Agriculture Hokkaido University
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Tomita Fusao
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Tomita Fusao
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Yokota A
Hokkaido Univ. Hokkaido Jpn
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Terasawa Yukio
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Wada Masaru
Lab. Microb. Physiol. Grad. Sch. Agric. Hokkaido Univ.
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