Development of Internal Transcribed Spacer Regions Amplification Restriction Fragment Length Polymorphism Methhod and Its Application in Monitoring the Population of Zygosaccharomyces rouxii M2 in Miso Fermentation
スポンサーリンク
概要
- 論文の詳細を見る
Recently, the use of the dry yeast of Zygosaccharomyces rouxii M2 for miso (soybean paste) fermentation has been established. A molecular monitoring method was developed and validated in this study to analyze the population of Z. rouxii M2 during the fermentation. The method was based on the restriction patterns of internal transcribed spacer (ITS) regions of the rDNA using HaeIII and HhaI. Among the homologous ITS regions of Z. rouxii strains, Z. rouxii M2 produced diagnostic bands by which it can be differentiated from the other strains used. The specific restriction bands were due to the difference in nucleotide sequence of two different copies of ITS of Z. rouxii M2. Both ITS copies showed 94% sequence similarity but a 13-bp nucleotide substitution and a 19-bp deletion were found in the ITS1 region. Phylogenetic trees were constructed based on ITS and 18S rDNA sequences and it was found that the ITS sequences provide better resolution for the classification of Z. rouxii M2. Since Z. rouxii M2 is a promising strain for use in miso fermentation as a dry starter, the method developed is significant in terms of industrial application in monitoring the growth of Z. rouxii M2 in miso fermentation.
- 社団法人日本生物工学会の論文
- 2003-11-25
著者
-
Yokota Atsushi
Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University
-
Asano Kozo
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
-
TOMITA FUSAO
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
-
SUJAYA INENGAH
Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University
-
SUJAYA I
Laboratory of Applied Microbiology, the Graduate School of Agriculture, Hokkaido University
-
冨田 房男
北海道大学農学部
-
TAMURA YOSHIFUMI
Hokkaido Food Processing Research Center
-
TANAKA TAKAYUKI
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
-
YAMAKI TAZUSA
Hokkaido Food Processing Research Center
-
IKEDA TAKAYUKI
Department of Food Science and Human Nutrition, Faculty of Human Ecology, Fuji Woman's University
-
KIKUSHIMA NAO
Hishiroku Co Ltd
-
YATA HIROSHI
Research Center, Nippon Beet Sugar Mfg, Co, Ltd
-
Sujaya Inengah
Northern Advancement Center For Science And Technology
-
Yokota Atsushi
Lab. Of Microbial Physiology Res. Fac. Of Agriculture Hokkaido Univ. Kita 9 Nishi 9 Kita-ku Sapporo
-
横田 篤
北大 大学院農学研究院
-
Sujaya I-nengah
Department Of Bioscience And Chemistry Graduate School Of Agriculture Hokkaido University
-
Yokota A
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
-
Yokota Atsushi
Hokkaido Univ. Sapporo Jpn
-
Yokota Atsushi
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
-
Asano Kozo
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
-
Asano Kozo
北海道大学農学部応用生命科学専攻
-
Tomita Fusao
Hokkaido University:hokkaido Study Center University Of The Air
-
Tomita Fusao
Laboratory Of Applied Microbiology
-
Tomita Fusao
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
-
Tomita Fusao
Department Of Agricultural Biochemistry Faculty Of Agriculture Hokkaido University
-
Tomita Fusao
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
-
Tamura Yoshifumi
Hokkaido Food Process Research Center
-
Yokota A
Hokkaido Univ. Hokkaido Jpn
-
Yata Hiroshi
Research Center Nippon Beet Sugar Mfg Co Ltd
-
Yamaki T
Hokkaido Food Processing Research Center
-
Ikeda Takayuki
Department Of Food Science And Human Nutrition Faculty Of Human Ecology Fuji Woman's University
-
Ikeda Takayuki
Department Of Cardiology Tsuruga Municipal Hospital
-
Ikeda Takayuki
The First Department Of Internal Medicine Kanazawa University
-
Tanaka T
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
-
Asano Kozo
Laboratory Of Applied Microbiology Graduate Faculty Of Agriculture Hokkaido University
関連論文
- Enzymatic synthesis of Mycoplasma fermentans specific glycoglycerophospholipid from 1,2-dipalmitoylglycerol(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Variation of Genes Encoding GGPLs Syntheses among Mycoplasma fermentans Strains
- One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.
- Characterization of an insertion sequence-like element, ISBlo15, identified in a size-increased cryptic plasmid pBK283 in Bifidobacterium longum BK28(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Determination of Enzymes from Colletotrichum sp. AHU9748 Essential for Lepidimoide Production from Okra Polysaccharide(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine.
- Biodesulfurization of Alkylated Forms of Dibenzothiophene and Benzothiophene by Sphingomonas subarctica T7b(ENVIRONMENTAL BIOTECHNOLOGY)
- Effects of long-term ingestion of difructose anhydride III (DFA III) on intestinal bacteria and bile acid metabolism in humans
- Effects of difructose anhydride III (DFA III) administration on bile acids and growth of DFA III-assimilating bacterium Ruminococcus productus on rat intestine.
- Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
- The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
- rDNA ITS Sequence of Rhizopus oryzae : Its Application to Classification and Identification of Lactic Acid Producers(Microbiology & Fermentation Technology)
- Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces
- (96) イネいもち病菌の非病原性遺伝子AVR-Piaのクローニング(平成20年度日本植物病理学会大会講演要旨)
- (107) イネいもち病菌の非病原性遺伝子Avr-Piaのクローニング(平成19年度日本植物病理学会大会講演要旨)
- (60) イネいもち病菌の非病原性遺伝子Avr-Piaのクローニング(平成18年度日本植物病理学会大会講演要旨)
- (2)イネいもち病菌ミニクロモソームの解析 : (1)反復配列の解析(平成16年度日本植物病理学会大会講演要旨)
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Production of Lepidimoide by an Endophytic Fungus from Polysaccharide Extracted from Abelmoschus sp. : Identification of the Product and the Organism Producing It
- Endophytes as Producers of Xylanase
- Isolation, Screening and Phylogenetic Identification of Endophytes from Plants in Hokkaido Japan and Java Indonesia
- 2Ep16 高発現変異型DegQ遺伝子の導入による組換えBacillus subtilisを用いたDFA IV直接生産法の改善(発酵生理学,発酵工学,一般講演)
- 2D17-4 Levan fructotransferaseのBacillus subtilis 168 における発現と単一培養系によるスクロースからのDFA IV生産(発酵生理学・発酵工学,一般講演)
- 450 有用な植物内生菌の探索とrDNA解析による分類と同定
- (16)イネいもち病菌のDNA型トランスポゾンOccanの解析(平成15年度日本植物病理学会大会講演要旨)
- (74)いもち病菌日本産菌株における非病原性遺伝子Avr-pitaの変異の解析(平成14年度 日本植物病理学会大会講演要旨)
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
- バイオサイエンス・バイオテクノロジーを担うもの
- 406 乳酸生成型Rhizopus oryzaeの選択的PCRによる迅速選抜法(分類・系統・遺伝学,一般講演)
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
- 568 大腸菌を用いた2-ヒドロキシ-4-フェニル酪酸(HPB)生産におけるNADPH再生系の確立(酵素・酵素工学・タンパク質工学,一般講演)
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Alterations of cellular physiology in Escherichia coli in response to oxidative phosphorylation impaired by defective F1-ATPase
- Isolation of a Cadmium-releasing Bacterium and Characterization of Its Novel Protease
- Development of Internal Transcribed Spacer Regions Amplification Restriction Fragment Length Polymorphism Methhod and Its Application in Monitoring the Population of Zygosaccharomyces rouxii M2 in Miso Fermentation
- Enhancement of Glucose Metabolism a Pyruvic Acid-Hyperproducing Escherichia coli Mutant Defective in F_1-ATPase Activity
- Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- The molecular phylogeny of the genus Rhizopus based on rDNA sequences
- The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
- カルシウム濃度および pH が Difructose anhydride III のラット腸粘膜カルシウム吸収充進作用に与える影響
- (75)変異株を利用したいもち病菌非病原性遺伝子のクローニング(平成14年度 日本植物病理学会大会講演要旨)
- 日本産いもち病菌菌株のDNAフィンガープリントおよび電気泳動核型解析
- 座談会 JBAのこれまでの10年, これからの10年を語る(第2部)
- 座談会 JBAのこれまでの10年, これからの10年を語る(第1部)
- Design and Test of a Lens and Reflector System of an Electrostatic Ion Trap for Molecular Physics
- 2J14-3 Sphingomonas subarctica T7b 株による種々の Alkyl BT, Alkyl DBT 類縁体の脱硫活性
- 酵素反応によってイヌリンより生産されたジフルクトース-アンヒドリドIIIの物理的, 化学的および生理学的性質
- 411 味噌モロミ由来の耐塩性酵母の分類学的多様性(分類・系統・遺伝学,一般講演)
- 711 Phytase生産量の高いAspergillus niger M3株の生育能力を高める研究(生物情報工学,発酵・発酵工学,一般講演)
- 味噌もろみ中のZygosaccharomyces rouxii M2株の菌数迅速モニタリング法の開発
- 乳酸菌の機能と新しい応用
- Comparative Effect of Repeated Ingestion of Difructose Anhydride III and Palatinose on the Induction of Gastrointestinal Symptoms in Humans
- Effect of Difructose Anhydride III on Calcium Absorption in Humans
- Evidence Suggesting That Difructose Anhydride III Is an Indigestible and Low Fermentable Sugar during the Early Stages after Ingestion in Humans
- (48)イネいもち病菌Magnaporthe griseaのセリンプロテアーゼ遺伝子の取得
- Metabolism of DFA III by Arthrobacter sp. H65-7 : Purification and Properties of a DFA III Hydrolysis Enzyme (DFA IIIase)
- Industrial production of difructose anhydride III(DFA III) from crude inulin extracted from chicory roots using Arthrobacter sp. H65-7 fructosyltransferase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effects of Indigenous Starter Cultures on the Microbial and Physicochemical Characteristics of Urutan, a Balinese Fermented Sausage(BREWING AND FOOD TECHNOLOGY)
- Enhanced Glutamic Acid Production by a H+-ATPase-Defective Mutant of Corynebacterium glutamicum
- Molecular Cloning of the Gene Encoding the di-D-Fructofuranose 1,2':2,3' Dianhydride Hydrolysis Enzyme (DFA IIlase) from Arthrobacter sp. H65-7
- Characterization of a Mutant of Lactococcus lactis with Reduced Membranebound ATPase Activity under Acidic Conditions
- Acid Sensitivity of a Mutant of Lactococcus lactis subsp. lactis C2 with Reduced Membrane-bound ATPase Activity
- Identification of a Putative Vacuolar Serine Protease Gene in the Rice Blast Fungus, Magnaporthe grisea(Biochemistry & Molecular Biology)
- Establishment of a New Cross of the Rice Blast Fungus Derived from Japanese Differential Strain Ina168 and Hermaphroditic Rice Pathogen Guy11(Microbiology & Fermentation Technology)
- Molecular Structure of rDNA Repeat Unit in Magnaporthe grisea
- Potential of Selected Strains of Lactic Acid Bacteria to Induce a Th1 Immune Profile
- L-Tryptophan Production by a Pyruvic Acid-Producing Escherichia coli Strain Carrying the Enterobacter aerogenes Tryptophanase Gene
- Enzymatic Synthesis of L-Tryptophan by Enterobacter aerogenes Tryptophanase Highly Expressed in Escherichia coli, and Some Properties of the Purified Enzyme
- Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry
- Molecular Cloning and Analysis of a Lipase Gene from Pseudomonas fluorescens No.33
- Pyruvic Acid Production by an F_1-ATPase-defective Mutant of Escherichia coli W1485lip2
- NP-101A, Antifungal Antibiotic from Streptomyces aurantiogriseus NPO-101
- Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
- Change of Proteins in Instant Chinese Noodle by the Fermentation of Lactobacillus plantarum NRIC 0380 Affects the Noodle Quality
- Identification and Characterization of a Karyotypic Mutation in Magnaporthe grisea
- Host Species-specific Repetitive DNA Sequence in the Genome of Magnaporthe grisea, the Rice Blast Fungus
- Production of Levanbiose by a Levan-degrading Enzyme from Streptomyces exfoliatus F3-2
- Induction and Catabolite Repression Mechanisms of Cellulase in Fungi
- A Novel Screen for the Detection of Chitin Acting Antifungal Compounds
- Bacterial Adjunct to Improve the Quality of Alkaline Noodles
- Microbial Demetallization of Crude Oil : Nickel Protoporphyrin Disodium as a Model Organo-Metallic Substrate
- Molecular Cloning and High-Level Expression in Escherichia coli of Fungal α-Galactosidase from Absidia corymbifera IFO 8084
- Effects of DFA IV in Rats : Calcium Absorption and Metabolism of DFA IV by Intestinal Microorganisms
- Molecular Cloning of Levan Fructotransferase Gene from Arthrobacter nicotinovorans GS-9 and Its Expression in Escherichia coli
- Purification of Levan Fructotransferase from Arthrobacter nicotinovorans GS-9 and Production of DFA IV from Levan by the Enzyme
- Molecular Cloning of an Inulin Fructotransferase (Depolymerizing) Gene from Arthrobacter sp. H65-7 and Its Expression in Escherichia coli
- Identification of the Cellobiose 2-Epimerase Gene in the Genome of Bacteroides fragilis NCTC 9343
- Difructose Anhydrides : Their Mass-Production and Physiological Functions
- Potential of Selected Strains of Lactic Acid Bacteria to Induce a Th1 Immune Profile
- Technological Advances in Bifidobacterial Molecular Genetics : Application to Functional Genomics and Medical Treatments