Enhanced Glutamic Acid Production by a H+-ATPase-Defective Mutant of Corynebacterium glutamicum
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概要
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Previously we reported that a mutant of Corynebacterium glutamicum ATCC14067 with reduced H+-ATPase activity, F172-8, showed an approximately two times higher specific rate of glucose consumption than the parent, but no glutamic acid productivity under the standard biotin-limited culture conditions, where biotin concentration was set at 5.5 μg/l in the production medium (Sekine et al., Appl. Microbiol. Biotechnol., 57, 534–540 (2001)). In this study, various culture conditions were tested to check the glutamic acid productivity of strain F172-8. The mutant was found to produce glutamic acid under exhaustive biotin limitation, where the biotin concentration of the medium was set at 2.5 μg/l with much smaller inoculum size. When strain F172-8 was cultured under the same biotin-limited conditions using a jar fermentor, 53.7 g/l of glutamic acid was produced from 100 g/l glucose, while the parent produced 34.9 g/l of glutamic acid in a medium with 5.5 μg/l biotin. The glutamic acid yield of strain F172-8 also increased under Tween 40-triggered production conditions (1.2-fold higher than the parent strain). The amounts of biotin-binding enzymes were investigated by Western blot analysis. As compared to the parent, the amount of pyruvate carboxylase was lower in the mutant; however, the amount of acetyl-CoA carboxylase did not significantly change under the glutamic acid production conditions. To the best of our knowledge, this is the first report showing that the H+-ATPase-defective mutant of C. glutamicum is useful in glutamic acid production.
- 社団法人 日本農芸化学会の論文
著者
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Yokota Atsushi
Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University
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Takesue Nobuchika
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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AOKI Ryo
Laboratory of Cellular Biochemistry, Division of Applied Life Sciences, Graduate School of Agricultu
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Yokota Atsushi
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Wada Masaru
Hokkaido Univ. Sapporo Jpn
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Aoki Ryo
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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TANAKA Kenji
Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya Univers
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Wada Masaru
Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University
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Aoki Ryo
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
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Takesue Nobuchika
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Takesue Nobuchika
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
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Tanaka Kenji
Laboratory Of Medical Mycology Research Institute For Disease Mechanism And Control Nagoya Universit
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Wada Masaru
Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University
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YOKOTA Atsushi
Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University
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AOKI Ryo
Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University
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TAKESUE Nobuchika
Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University
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TANAKA Kenji
Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University
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