Mechanism of increased respiration in an H^+-ATPase-defective mutant of Corynebacterium glutamicum(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• We previously reported that a spontaneous H^+-ATPase-defective mutant of Corynebacterium glutamicum, F172-8, derived from C. glutamicum ATCC 14067, showed enhanced glucose consumption and respiration rates. To investigate the genomebased mechanism of enhanced respiration rate in such C. glutamicum mutants, A-1, an H^+-ATPase-defective mutant derived from C. glutamicum ATCC 13032, which harbors the same point mutation as F172-8, was used in this study. A-1 showed similar fermentation profiles to F172-8 when cultured in a jar fermentor. Enzyme activity measurements, quantitative real-time PCR, and DNA microarray analysis suggested that A-1 enhanced malate:quinone oxidoreductase/malate dehydrogenase and L-lactate dehydrogenase/NAD^+-dependent-lactate dehydrogenase coupling reactions, but not NADH dehydrogenase-II, for reoxidation of the excess NADH arising from enhanced glucose consumption. A-1 also up-regulated succinate dehydrogenase, which may result in the relief of excess proton-motive force (pmf) in the H^+-ATPase mutant. In addition, the transcriptional level of cytochrome bd oxidase, but not cytochrome bc_1-aa_3, also increased, which may help prevent the excess pmf generation caused by enhanced respiration. These results indicate that C. glutamicum possesses intriguing strategies for coping with NADH over-accumulation. Furthermore, these mechanisms are different from those in Escherichia coli, even though the two species use similar strategies to prevent excess pmf generation.

著者

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• 横田 篤

  北大農

• Yokota Atsushi

  Laboratory Of Bioorganic Chemistry

• Yokota A

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Yokota Atsushi

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Yokota Atsushi

  Hokkaido Univ. Sapporo Jpn

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Wada Masaru

  Hokkaido Univ. Sapporo Jpn

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Yokota Atsushi

  Lab. Microb. Physiol. Grad. Sch. Agric. Hokkaido Univ.

• Li Liyuan

  Lab. Microb. Physiol. Grad. Sch. Agric. Hokkaido Univ.

• Wada Masaru

  Lab. Microb. Physiol. Grad. Sch. Agric. Hokkaido Univ.

• Wada Masaru

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Sawada Kazunori

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• Kato Yui

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• Imai Keita

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• Li Liyuan

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• Kato Yui

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Li Liyuan

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Sawada Kazunori

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Imai Keita

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

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