Purification and Characterization of a Novel Polysaccharide Involved in the Pellicle Produced by a Thermotolerant Acetobacter Strain(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Acetobacter strains able to produce a thick pellicle at 37℃ were screened among many thermotolerant strains isolated from fruits in Thailand. As a result, Acetobacter sp. SKU 1100 was selected as the producer of a relatively thick pellicle even when cultured at higher temperatures such as 37℃ or 40℃. This strain could produce a pellicle polysaccharide in a shaking submerged culture as well as under static culture conditions. The polysaccharide was found to be attached to the bacterial cells. Although the polysaccharide production was higher at 30℃ than at 37℃ in shaking submerged culture, the productivity in static culture was not decreased even at higher temperatures. The membrane-attached polysaccharide was purified from the SKU 1100 strain by cell disruptions using either ultrasonic treatment or lysozyme treatment, followed by ultracentrifugation, enzyme treatments, dialysis against SDS, DEAE-cellulose column chromatography, alcohol precipitation, and gel filtration chromatography. The polysaccharide purified by the sonic treatment and also by the mild conditions using lysozyme treatment had the same average molecular mass of 120 kDa. The purified polysaccharide was composed of three different monosaccharides ; glucose, galactose, and rhamnose, in an approximately equimolar ratio of 1 : 1 : 1.
- 社団法人日本農芸化学会の論文
- 2002-04-23
著者
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足立 収生
山口大学農学部生物機能科学科
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足立 収生
山口大・農・生物機能
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Theeragool G.
カセサート大学理
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TOYAMA Hirohide
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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THEERAGOOL Gunjana
Department of Microbiology, Faculty of Science, Kasetsart University
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LOTONG Napha
Department of Microbiology, Faculty of Science, Kasetsart University
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MATSUSHITA Kazunobu
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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ADACHI Osao
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Toyama Hirohide
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Adachi Osao
Department Of Agricultural Chemistry Yamaguchi University
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Lotong Napha
Department Of Microbiology Faculty Of Science Kasetsart University
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Lotong Napha
カセサート大理 微生物
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MOONMANGMEE SOMPORN
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Theeragool G.
カセサート大理微生物
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Theeragool Gunjana
カセサート大理 微生物
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Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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足立 収生
山口大学 農
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足立 収生
山口大・農化
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Matsushita K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Theeragool Gunjana
Department Of Microbiology Faculty Of Science Kasetsart University
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松下 一信
山口大 農
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Moonmangmee Somporn
Department Of Biotechnology Thailand Institute Of Scientific And Technological Research
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Lotong Napha
Department Of Microbioloby Faculty Of Science Kasetsart University
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Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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