A Novel Polysaccharide Involved in the Pellicle Formation of Acetobacter aceti

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概要

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• Acetobacter aceti IFO 3284 has been shown to have two types of strains: one forms a smooth- surfaced colony (S strain) and the other forms a rough-surfaced colony (R strain) (Matsushita et al., 1992). In this study, both S and R strains were isolated and characterized. The S strain grew well in submerged culture but very poorly in static culture. In contrast, the R strain grew well in static culture by floating on the surface of the culture medium, as well as in shaking submerged culture. Scanning electron microscopy revealed that the R strain was covered by some amorphous materials that were not seen in the S strain. The R strain produced 5-fold higher levels of sugars related to polysaccharides responsible for pellicle formation than the S strain did. Unlike cellulose of Acetobacter xylinum, the polysaccharides of the R strain were cellulase-resistant and alkaline- sensitive. The polysaccharides were not secreted into the culture medium, and more than 90% of them were retained in the membrane fraction when the cells were disrupted under mild conditions by lysozyme treatment. Furthermore, the polysaccharides were shown to be mainly attached to the outer membrane when separated. After solubilization with β-octylglucoside, the membrane-attached polysaccharides were purified by several steps including enzyme treatment, column chromatography and alcohol precipitation. The purified polysaccharide was estimated to have an apparent molecular mass of 700-kDa based on Sephacryl S-500 column chromatography, and to be composed of two monosaccharides, glucose and rhamnose, at an approximately equimolar ratio. Thus, in this study, we clarified that the A. aceti R strain produced a polysaccharide associated with the flotation of the cells on the medium surface, like A. xylinum, and that the polysaccharide was a novel one consisting of glucose and rhamnose.

• 社団法人日本生物工学会の論文
• 2002-02-25

著者

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• Toyama Hirohide

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• TANASUPAWAT Somboon

  Chulalongkon大学 薬学部 微生物学科

• KAWABATA Koji

  Department of Physical Sciences, Hiroshima University

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• Kawabata Koji

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• MOONMANGMEE SOMPORN

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• TANAKA SHUHEI

  Department of Plant Pathology, Faculty of Agriculture, Yamaguchi University

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Tanasupawat Somboon

  Dep. Of Biochemistry And Microbiology Fac. Of Pharmaceutical Sciences Chulalongkorn Univ.

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Tanaka S

  Dep. Of Biochemistry And Microbiology Fac. Of Pharmaceutical Sciences Chulalongkorn Univ.

• 松下 一信

  山口大 農

• Tanaka Shuhei

  Department Of Biological And Environmental Sciences Faculty Of Agriculture Yamaguchi University

• Moonmangmee Somporn

  Department Of Biotechnology Thailand Institute Of Scientific And Technological Research

• Kawabata Koji

  Department Of Astronomy Kyoto University

• Tanaka Shuhei

  Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

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