Purification and Properties of Two Different Dihydroxyacetone Reductases in Gluconobacter suboxydans Grown on Glycerol
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概要
- 論文の詳細を見る
It is well known that in oxidative fermentation microbial growth is improved by the addition of glycerol. In a wild strain, glycerol was converted rapidly to dihydroxyacetone (DHA) quantitatively in the early growth phase by the action of quinoprotein glycerol dehydrogenase (GLDH), and then DHA was incorporated into the cells by the early stationary phase. Two DHA reductases (DHARs), NADH-dependent (NADH-DHAR) (EC 1.1.1.6) and NADPH-dependent (NADPH-DHAR) (EC 1.1.1.156), were detected in the same cytoplasm of Gluconobacter suboxydans IFO 3255. The former appeared to be inducible and labile in nature while the latter was constitutive and stable. The two DHARs were separated each other and were finally purified to crystalline enzymes. This report might be the first one dealing with NADPH-DHAR that has been crystallized. The two DHARs were specific only to DHA reduction to glycerol and thus contributed to cytoplasmic DHA metabolism, resulting in an improved biomass yield with the addition of glycerol.
- 社団法人 日本農芸化学会の論文
- 2008-08-23
著者
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品川 恵美子
宇部高専物質工学科
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ADACHI Osao
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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ANO Yoshitaka
Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University
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Murata K
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Murata K
Laboratory Of Basic And Applied Molecular Biotechnology Division Of Food Science And Biotechnology G
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Murata Kousaku
Div. Of Food Sci. And Biotech. Grad. Sch. Of Agriculture Kyoto Univ.
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Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Adachi Osao
Department Of Agricultural Chemistry Yamaguchi University
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Shinagawa Emiko
Department Of Chemical And Biological Engineering Ube National College Of Technology
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Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Murata Kousaku
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Matsushita K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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松下 一信
山口大 農
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Adachi O
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Ano Yoshitaka
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Shinagawa Emiko
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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ANO Yoshitaka
Department of Applied Bioscience, Faculty of Agriculture, Ehime University
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