Crystallization and Properties of NADPH-Dependent L-Sorbose Reductase from Gluconobacter melanogenus IFO 3294

スポンサーリンク

概要

• 論文の詳細を見る

• NADPH-Dependent L-sorbose reductase (SORD), synonimously NADP-dependent D-sorbitol dehydrogenase) was purified and crystallized for the first time from the cytosolic fraction of Gluconobacter melanogenus IFO 3294. The enzyme catalyzed oxidoreduction between D-sorbitol and L-sorbose in the presence of NADP or NADPH. Affinity chromatography by a Blue-dextran Sepharose 4B column was effective for purifying the enzyme giving about 770-fold purification with an overall yield of more than 50%. The crystalline enzyme showed a single sedimentation peak in analytical ultracentrifugation, giving an apparent sedimentation constant of 3.8s. Gel filtration on a Sephadex G-75 column gave the molecular mass of 60 kDa to the enzyme, which dissociated into 30 kDa subunit on SDSPAGE, indicating that the enzyme is composed of 2 identical subunits. Reduction of L-sorbose to D-sorbitol predominated in the presence of NADPH with the optimum pH of 5.0-7.0. Oxidation of D-sorbitol to L-sorbose was observed in the presence of NADP at the optimum pH of 7.0-9.0. The relative rate of L-sorbose reduction was more than seven times higher to that of D-sorbitol oxidation. NAD and NADH were inert for both reactions. D-Fructose reduction in the presence of NADPH did not occur with SORD. Since the reaction rate in L-sorbose reduction highly predominated over D-sorbitol oxidation over a wide pH range, the enzyme could be available for direct enzymatic measurement of L-sorbose. Even in the presence of a large excess of D-glucose and other substances, oxidation of NADPH to NADP we highly specific and stoichiometric to the L-sorbose reduced. Judging from the enzymatic properties, SORD would contribute to the intracellular assimilation of L-sorbose incorporated from outside the cells where L-sorbose is accumulated in huge amounts in the culture medium.

• 社団法人日本農芸化学会の論文
• 1999-12-23

著者

• 品川 恵美子

  宇部高専物質工学科

• Moonmangmee Duangtip

  山口大農応用生物化学

• Theeragool G.

  カセサート大学理

• MOONMANGMEE Duangtip

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• TOYAMA Hirohide

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• THEERAGOOL Gunjana

  Department of Microbiology, Faculty of Science, Kasetsart University

• LOTONG Napha

  Department of Microbiology, Faculty of Science, Kasetsart University

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University

• SHINAGAWA Emiko

  Department of Chemical and biological Engineering, Ube National College of Technology

• Toyama Hirohide

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• Lotong Napha

  Department Of Microbiology Faculty Of Science Kasetsart University

• Lotong Napha

  カセサート大理 微生物

• Shinagawa Emiko

  Department Of Chemical And Biological Engineering Ube National College Of Technology

• Theeragool G.

  カセサート大理微生物

• Theeragool Gunjana

  カセサート大理 微生物

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Moonmangmee Duangtip

  Department Of Microbiology Faculty Of Science King Mongkut's University Of Technology Thonburi

• Moonmangmee Duangtip

  山口大農 生物機能科学

• 足立 収生

  山口大・農化

• Theeragool Gunjana

  Department Of Microbiology Faculty Of Science Kasetsart University

• Ano Yoshitaka

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Lotong Napha

  Department Of Microbioloby Faculty Of Science Kasetsart University

• Shinagawa Emiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Applied Bioscience, Faculty of Agriculture, Ehime University

関連論文

• 10.酢酸菌のグルコン酸酸化にかかわる第3の膜酵素・FADグルコン酸脱水素酵素(第419回研究協議会研究発表要旨,ビタミンB研究委員会)
• L-Erythrulose Production by Oxidative Fermentation is Catalyzed by PQQ-Containing Membrane-bound Dehydrogenase(Microbiology & Fermentation Technology)
• Purification and Characterization of Membrane-bound Quinoprotein Cyclic Alcohol Dehydrogenase from Gluconobacter frateurii CHM 9(Microbiology & Fermentation Technology)
• Isolation and Characterization of Thermotolerant Gluconobacter Strains Catalyzing Oxidative Fermentation at Higher Temperatures
• Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
• 5. 酢酸菌による環状アルコールの酸化
• 2.酢酸菌による糖質酸化と補酵素
• 5. 酢酸菌による糖質酸化と補酵素
• 高温耐性酢酸菌の検索と食酢製造への応用 : 微生物
• Acetobacter属酢酸菌に特徴的な酢酸の過酸化に関する研究(第1報) : 微生物
• Molecular Target of Piperine in the Inhibition of Lipid Droplet Accumulation in Macrophages(Biochemistry)
• Disruption of the Membrane-Bound Alcohol Dehydrogenase-Encoding Gene Improved Glycerol Use and Dihydroxyacetone Productivity in Gluconobacter oxydans
• Molecular Properties of Membrane-Bound FAD-Containing D-Sorbitol Dehydrogenase from Thermotolerant Gluconobacter frateurii Isolated from Thailand
• 3-Dehydroquinate Production by Oxidative Fermentation and Further Conversion of 3-Dehydroquinate to the Intermediates in the Shikimate Pathway(Microbiology & Fermentation Technology)
• Purification and Characterization of Membrane-bound Quinoprotein Quinate Dehydrogenase(Biochemistry & Molecular Biology)
• Molecular Cloning of Quinohemoprotein Alcohol Dehydrogenase, ADH IIB, from Pseudomonas putida HK5(Biochemistry & Molecular Biology)
• Membrane-bound Quinoprotein D-Arabitol Dehydrogenase of Gluconobacter suboxydans IFO 3257 : A Versatile Enzyme for the Oxidative Fermentation of Various Ketoses(Microbiology & Fermentation Technology)
• Alterations of cellular physiology in Escherichia coli in response to oxidative phosphorylation impaired by defective F1-ATPase
• Methionaquinone is a Direct Natural Electron Acceptor for the Membrane-bound Hydrogenase in Hydrogenobacter thermophilus Strain TK-6(Biological Chemistry)
• Total Synthesis of Malformin C, an Inhibitor of Bleomycin-Induced G2 Arrest
• 1S-Ep05 耐熱性酢酸菌を使った酸化発酵による有用物質生産系の開発(伝統的醗酵微生物の新しい利用展開,シンポジウム,伝統の技と先端科学技術の融合)
• Production of 4-Keto-D-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens
• Biochemical and Spectroscopic Properties of Cyanide-Insensitive Quinol Oxidase from Gluconobacter oxydans
• Guadinomines, Type III Secretion System Inhibitors, Produced by Streptomyces sp. K01-0509 : II. Physico-chemical Properties and Structure Elucidation
• Guadinomines, Type III Secretion System Inhibitors, Produced by Streptomyces sp. K01-0509 : I. Taxonomy, Fermentation, Isolation and Biological Properties
• A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
• 琉球大学農学部「亜熱帯生物資源科学科,発酵・生命科学コース」と,設置予定の「泡盛学寄附講座」の紹介(九州支部,Branch Spirit)
• 2P030 酢酸菌Gluconobacter frateurii由来L-sorbose reductaseのX線結晶構造解析(蛋白質-構造機能相関,第48回日本生物物理学会年会)
• Preparation and Preservation of Freeze-dried Cells of Acetic Acid Bacteria with Aldehyde Oxidase Activity
• A Novel Polysaccharide Involved in the Pellicle Formation of Acetobacter aceti
• Citrinamides, New Potentiators of Antifungal Miconazole Activity, Produced by Penicillium sp. FKI-1938
• Tensyuic Acids, New Antibiotics Produced by Aspergillus niger FKI-2342
• Vanilin Formation by Microbial Amine Oxidases from Vanillylamine
• Anti-atherosclerotic Activity of Triacsin C, an Acyl-CoA Synthetase Inhibitor
• Siccanin Rediscovered as a Species-Selective Succinate Dehydrogenase Inhibitor
• Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol
• The Selectivity of Beauveriolide Derivatives in Inhibition toward the Two Isozymes of Acyl-CoA: cholesterol Acyltransferase
• Selective Inhibition of Acyl-CoA : cholesterol Acyltransferase 2 Isozyme by Flavasperone and Sterigmatocystin from Aspergillus Species
• Selectivity of Pyripyropene Derivatives in Inhibition toward Acyl-CoA : cholesterol Acyltransferase 2 Isozyme
• Characterization of Membrane-bound Spermidine Dehydrogenase of Citrobacter freundii
• Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans
• Quinoprotein Alcohol Dehydrogenase of Acetic Acid Bacteria: Kinetic Study on the Enzyme Purified from Acetobacter methanolicus
• Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144 : II. Biological Activities
• Cyslabdan, a New Potentiator of Imipenem Activity against Methicillin-resistant Staphylococcus aureus, Produced by Streptomyces sp. K04-0144 : I. Taxonomy, Fermentation, Isolation and Structural Elucidation
• Enzymes Responsible for Acetate Oxidation by Acetic Acid Bacteria
• Microbiological Aspects of Acetate Oxidation by Acetic Acid Bacteria, Unfavorable Phenomena in Vinegar Fermentation
• 白色腐朽菌Trametes sanguinea M85-2によるラッカーゼの生産 : 微生物
• Inhibition of Lipid Droplet Accumulation in Mouse Macrophages by Stemphone Derivatives
• Structure-activity Relationships of Stemphones, Potentiators of Imipenem Activity against Methicillin-resistant Staphylococcus aureus
• Purification and Characterization of Methylamine Oxidase Induced in Aspergillus niger AKU 3302
• NADPH Oxidase System as a Superoxide-generating Cyanide-Resistant Pathway in the Respiratory Chain of Corynebacterium glutamicum
• Role of Cytochrome c-553(CO), the Second Subunit of Alcohol Dehydrogenase, in the Azide-Insensitive Respiratory Chain and in Oxidative Fermentation of Gluconobacter Species
• Ethanol Oxidase Respiratory Chain of Acetic Acid Bacteria. Reactivity with Ubiquinone of Pyrroloquinoline Quinone-dependent Alcohol Dehydrogenases Purified from Acetobacter aceti and Gluconobacter suboxydans
• Formaldehyde Elimination with Formaldehyde and Formate Oxidase in Membrane of Acetic Acid Bacteria(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
• A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
• Involvement of Multiple Copper/Topa Quinone-Contanining and Flavin-Containing Amine Oxidases and NAD(P)^+ Aldehyde Dehydrogenases in Amine Degradation by Filamentous Fungi
• Azurin Involved in Alcohol Oxidation System in Pseudomonas putida HK5 : Expression Analysis and Gene Cloning(Biochemistry & Molecular Biology)
• Enzymatic Preparation of Metabolic Intermediates, 3-Dehydroquinate and 3-Dehydroshikimate, in the Shikimate Pathway
• Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production
• High Shikimate Production from Quinate with Two Enzymatic Systems of Acetic Acid Bacteria
• Conversion of Capsular Polysaccharide, Involved in Pellicle Formation, to Extracellular Polysaccharide by galE Deletion in Acetobacter tropicalis
• Growth Promoting Substance in Yeast Extract for Methylotrophic Growth of Candida boidinii
• A Novel Type of Formaldehyde-Oxidizing Enzyme from the Membrane of Acetobacter sp. SKU 14
• Electron Transfer Ability from NADH to Menaquinone and from NADPH to Oxygen of Type II NADH Dehydrogenase of Corynebacterium glutamicum
• Acetic Acid Fermentation of Acetobacter pasteurianus: Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation
• Quinoprotein Alcohol Dehydrogenase Is Involved in Catabolic Acetate Production, while NAD-Dependent Alcohol Dehydrogenase in Ethanol Assimilation in Acetobacter pasteurianus SKU1108(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Purification and Characterization of Two NAD-Dependent Alcohol Dehydrogenases (ADHs) Induced in the Quinoprotein ADH-Deficient Mutant of Acetobacter pasteurianus SKU1108(Microbiology & Fermentation Technology)
• Purification and Characterization of a Novel Polysaccharide Involved in the Pellicle Produced by a Thermotolerant Acetobacter Strain(Microbiology & Fermentation Technology)
• Purification and Characterization of Membrane-bound Malate Dehydrogenase from Acetobacter sp. SKU 14(Microbiology & Fermentation Technology)
• Membrane-bound Sugar Alcohol Dehydrogenase in Acetic Acid Bacteria catalyzes L-Ribulose Formation and NAD-Dependent Ribitol Dehydrogenase is Independent of the Oxidative Fermentation
• Crystallization and Properties of NADPH-Dependent L-Sorbose Reductase from Gluconobacter melanogenus IFO 3294
• Crystallization and Properties of NAD-Dependent D-Sorbitol Dehydrogenase from Gluconobacter suboxydans IFO 3257
• Development of Thermotolerant Acetic Acid Bacteria Useful for Vinegar Fermentation at Higher Temperatures
• Further Properties of Spermidine Dehydrogenase from Citrobacter freundii IFO 12681
• A Tightly Bound Quinone Functions in the Ubiquinone Reaction Sites of Quinoprotein Alcohol Dehydrogenase of an Acetic Acid Bacterium, Gluconobacter suboxydans
• A Novel 3-Dehydroquinate Dehydratase Catalyzing Extracellular Formation of 3-Dehydroshikimate by Oxidative Fermentation of Gluconobacter oxydans IFO 3244
• Energy Metabolism of a Unique Acetic Acid Bacterium, Asaia bogorensis, That Lacks Ethanol Oxidation Activity
• Distinct Physiological Roles of Two Membrane-Bound Dehydrogenases Responsible for D-Sorbitol Oxidation in Gluconobacter frateurii
• Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol
• Prosthetic Group of Aldehyde Dehydrogenase in Acetic Acid Bacteria Not Pyrroloquinoline Quinone
• Purification and Characterization of Membrane-Bound 3-Dehydroshikimate Dehydratase from Gluconobacter oxydans IFO 3244, A New Enzyme Catalyzing Extracellular Protocatechuate Formation
• Production of 4-Keto-D-arabonate by Oxidative Fermentation with Newly Isolated Gluconacetobacter liquefaciens
• Conversion of Quinate to 3-Dehydroshikimate by Ca-Alginate-Immobilized Membrane of Gluconobacter oxydans IFO 3244 and Subsequent Asymmetric Reduction of 3-Dehydroshikimate to Shikimate by Immobilized Cytoplasmic NADP-Shikimate Dehydrogenase
• Acetic Acid Fermentation of Acetobacter pasteurianus : Relationship between Acetic Acid Resistance and Pellicle Polysaccharide Formation
• Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans
• Preparation of Enzymes Required for Enzymatic Quantification of 5-Keto-D-gluconate and 2-Keto-D-gluconate
• Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
• Purification and Properties of Two Different Dihydroxyacetone Reductases in Gluconobacter suboxydans Grown on Glycerol
• The Occurrence of a Novel NADH Dehydrogenase, Distinct from the Old Yellow Enzyme, in Gluconobacter Strains
• 耐熱性酢酸菌を使った酸化発酵による有用物質生産系の開発(伝統的発酵微生物の新しい利用展開)
• Purification and Characterization of Laccase from White Rot Fungus Trametes sanguinea M85-2
• Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans
• Purification and Characterization of Fe(III)-EDTA Reductase from Bacillus sp. B-3
• Global Analysis of the Genes Involved in the Thermotolerance Mechanism of Thermotolerant Acetobacter tropicalis SKU1100
• Formation of 4-Keto-D-aldopentoses and 4-Pentulosonates (4-Keto-D-pentonates) with Unidentified Membrane-Bound Enzymes from Acetic Acid Bacteria
• Mechanism of increased respiration in an H^+-ATPase-defective mutant of Corynebacterium glutamicum(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Enzymatic Synthesis of 4-Pentulosonate (4-Keto-D-pentonate) from D-Aldopentose and D-Pentonate by Two Different Pathways Using Membrane Enzymes of Acetic Acid Bacteria
• 9.酢酸菌の五単糖類に対する新規な膜結合型酸化還元酵素の発見(第427回研究協議会研究発表要旨,ビタミンB研究委員会)
• Pentose Oxidation by Acetic Acid Bacteria Led to a Finding of Membrane-Bound Purine Nucleosidase

もっと見る 閉じる

スポンサーリンク