Further Properties of Spermidine Dehydrogenase from Citrobacter freundii IFO 12681
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概要
- 論文の詳細を見る
Spermidine dehydrogenase was crystallized for the first time from the cell-free extract of Citrobacter freundii IFO 12681. The enzyme contained one mole of heme b as the prosthetic group and the heme was autooxidizable. Absorption maxima in the visible region were at 561,530,428,and 335 nm with the reduced enzyme, while only one peak at 416nm predominated with the oxidized enzyme. No flavin prosthetic group was detected with the enzyme and PQQ was detected in acid hydrolysate of the enzyme. PQQ appeared to be bound covalently to the enzyme. Thus, the spermidine dehydrogenase of C. freundii has been characterized to be a quinohemoprotein. This is the first instance of amine dehydrogenase in which both heme b and PQQ are involved in enzyme activity.
- 社団法人日本農芸化学会の論文
- 1992-02-23
著者
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MATSUSHITA Kazunobu
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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ADACHI Osao
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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MURATA KOUSAKU
Research Institute for Food Science, Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura A
Kyoto Univ. Kyoto
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Kimura Akira
Research Institute For Food Science Kyoto University
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Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Adachi Osao
Department Of Agricultural Chemistry Yamaguchi University
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Kimura A
Hokkaido Univ. Sapporo Jpn
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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Kimura A
Research Institute For Food Science Kyoto University
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HISANO Tomohiro
Research Institute for Food Science, Kyoto University
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Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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