Safety Assessment of Genetically Engineered Food : Detection and Monitoring of Glyphosate-Tolerant Soybeans
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概要
- 論文の詳細を見る
A detection technique for the genetically engineered food, glyphosate-tolerant soybean (GTS), was designed. Commercial soybeans imported from North America were cultured in pots and genomic DNA was isolated from their leaves. To detect the genes, promoter and terminator, involved in the expression of glyphosate tolerance, PCR was done using the genomic DNA and chemically synthesized primers specific to the genes. DNAs with predicted sizes were amplified and confirmed by DNA sequencing to be the genes responsible for the expression of glyphosate tolerance. Glyphosate-tolerant soybeans were found to form approximately 1.1% of the commercial soybeans, when commercially available soybeans were cultivated and number of soybeans resistant to glyphosate was found. This level is somewhat lower than an estimated value announced officially on the basis of the cultivation area of the glyphosate-tolerant soybeans.
- 社団法人日本農芸化学会の論文
- 1998-07-23
著者
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橋本 渉
京大院・農・食生科
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MURATA KOUSAKU
Research Institute for Food Science, Kyoto University
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Murata K
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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KITO Makoto
Research Institute for Food Science, Kyoto University
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MOMMA Keiko
Research Institute for Food Science, Kyoto University
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HASHIMOTO Wataru
Research Institute for Food Science, Kyoto University
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OZAWA Sachiko
Research Institute for Food Science, Kyoto University
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UTSUMI Shigeru
Research Institute for Food Science, Kyoto University
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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SHIRAI Nobuaki
Industrial Research Center of Shiga Prefecture
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Hashimoto Wataru
Research Institute For Food Science Kyoto University
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Murata Kousaku
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Utsumi S
Division Of Agronomy And Horticultural Science Graduate School Of Agriculture Kyoto University
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Utsumi Shigeru
Research Institute For Food Science Kyoto University
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Momma Keiko
Laboratory Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers
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Kito M
Food Science Research Institute Fuji Oil Co. Ltd.
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Kito Makoto
Research Institute For Food Science Kyoto University
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Ozawa Sachiko
Research Institute For Food Science Kyoto University
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