Bacterial Alginate Lyase : Characterization of Alginate Lyase-Producing Bacteria and Purification of the Enzyme
スポンサーリンク
概要
- 論文の詳細を見る
Two kinds of bacteria producing alginate lyase were isolated from ditch sample and they were designated A-1 and A-2 strains. Morphological and physiological properties of the two strains were closely similar to each other, except that the cells of strain A-1 showed flocculation during growth on a medium containing alginate as a carbon source. The cells of strain A-1 produced three kinds of intracellular alginate lyases (A_1-I, A_1-II-_1 and A_1-II-_2). The cells of strain A-2 also contained three kinds of alginate lyases (A_2-I, A_2-II-_1 and A_2-II-_2) in cells. Completely purified A_1-II-_1 and A_2-I enzymes were monomer with a molecular weight of 60,000. A_1-I (partially purified), A_1-II-_1 and A_2-I enzymes were most active at pH 8.0 and hydrolyzed sodium-, potassium- and propylene glycol-alginates at almost the same efficiencies.
- 社団法人日本生物工学会の論文
- 1991-09-25
著者
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MURATA KOUSAKU
Research Institute for Food Science, Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura Akira
Research Institute For Food Science Kyoto University
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Okayama Kenichi
Otsuka Chemical Co. Ltd. Division Of Food And Beverage Research
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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Kimura A
Research Institute For Food Science Kyoto University
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YONEMOTO YOSHIMASA
Otsuka Chemical Co. Ltd., Division of Food & Beverage Research
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YAMAGUCHI HISAKO
Otsuka Chemical Co. Ltd., Division of Food & Beverage Research
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Yamaguchi Hisako
Otsuka Chemical Co. Ltd. Division Of Food & Beverage Research
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Yonemoto Yoshimasa
Food & Beverage Division Otsuka Chemical Co.
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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