Isolation of a Constitutive N-Acetylneuraminate Lyase-producing Mutant of Escherichia coli and Its Use for NPL Production
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概要
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An E. coli mutant (M8328) was isolated by NTG mutagenesis from E. coli KMS3207 which has an inducible N-acetylneuraminate lyase (NPL). The mutant was resistant to catabolite repression and produced NPL constitutively. To improve the productivity of NPL, a hybrid plasmid, pMK6, carrying the npl gene was introduced into the mutant, M8328. The NPL activity level was increased more than 5-fold in the pMK6-harboring mutant compared with that in the mutant, when the cells were grown in inducer (N-acetylneuraminic acid)-free medium. The NPLs produced by the mutant and the pMK6-harboring cells were structurally and immunologically identical with that purified from the parent strain (KMS3207).
- 社団法人 日本農芸化学会の論文
著者
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Murata Kousaku
Research Institute For Food Science Kyoto Univeristy
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Tsukada Yoji
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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Ohta Yasuhiro
Kyoto Research Laboratories Marukin Shoyu Co. Ltd
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Sugimori Tsunetake
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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