Preparation of UDP-Glucuronic Acid by Bacterial UDP-Glucose Dehydrogenase
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概要
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The distrivution of UDPG dehydrogenase in microorganisms, especially Bacillus species, was investigated and it was found that several strains of Bacillus licheniformis possessed this enzyme and were most suitable for the enzymatic preparation of UDPGA from UDPG since these organisms scarcely showed decomposition of both UDPG and UDPGA. Disintegrated cells such as sonicated ones can easily oxidize UDPG to UDPGA with a high convension rate. The reaction product was purified by a Dowex 1(Cl-form) column and was identified as UDPGA by chemical and physical analyses and by biological functions such as glucuronide formation with o-aminophenol by the homogenate of mouse liver.UDPG dehydrogenase of B. licheniformis required NAD for it's activity. The optimum pH is 9.5 and the optimum temperature is 40℃. Under aerobic conditions the NADH oxidizing system in this organism coupled with the dehydrogenation of UDPG facilitated the completion of UDPG-UDPGA conversion. In the presence of 0.5 mM NAD, 6 mM UDPG was dehydrogenated to UDPGA at a conversion rate of 87% 17 hr at 30℃ by the sonicate of B. Licheniformis IAM 11054.
- 公益社団法人日本生物工学会の論文
- 1977-10-25
著者
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Tsukada Yoji
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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Tazuke Yasuhiko
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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Sugimori Tsunetake
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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