Production, Purification and Characterization of HYI, an Antiyeast Substance, Produced by Hansenula saturnus
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概要
- 論文の詳細を見る
An anti-yeast substance, HYI, was produced by Hansenula saturnus. Production of HYI under aerobic incubation conditions took place in parallel with yeast cellular growth. HYI was concentrated by ultra filtration from the culture filtrate and purified by ion-exchange chromatography followed by gel filtration. It was then crystallized. HYI migrated as a single protein band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis and had a molecular weight of about 8500. Anthrone positive compounds could not be detected in HYI. The isoelectric point ofHYI was pH 5.80±0.05. The activity ofHYI was unaffected by heating at 60°C for 1 hr. It showed a broad anti-yeast spectrum.
- 社団法人 日本農芸化学会の論文
著者
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Tsukada Yoji
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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Ohta Yasuhiro
Kyoto Research Laboratories Marukin Shoyu Co. Ltd
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Sugimori Tsunetake
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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SUGIMORI Tsunetake
Kyoto Research Laboratories, Marukin Shoyu Co., Ltd.
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