Inhibition in Vitro Fertilization of Mouse Gametes by Sulfated Sialic Acid Polymers
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概要
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The effect of sialic acid (N-acetyl neuraminic acid), sialic acid dimer, sialic acid polymers (colominic acid) and sulfated colominic acid on the activity of hyaluronidase, on the dispersion of cumulus cells by mouse sperm and on in vitro mouse fertilization (sperm penetration of zona pelllucida) were evaluated. Bovine testicular hyaluronidase activity was significantly inhibited by colominic acid and sulfated colominic acid, but not by sialic acid and its dimer. The dispersion of cumulus cells from eggs by mouse sperm was also inhibited by colominic acid and sulfated colominic acid. In vitro fertilization of mouse gametes was inhibited by sulfated colominic acid. The IC_<50> value of sulfated colominic acid-induced inhibition of fertilization was 0.3 mg/ml (ca. 0.9 mM). The value changed from 0.9 mM for cumulus-surronded egg to 1.5 mM for cumulus free-egg. On the other hand, colominic acid showed little or no inhibitory effect on mouse in vitro fertilization at 0.5 mg/ml (ca. 1.6 mM). This antifertility activity by sulfated colominic acid did not appear to be due to an effect on sperm motility or on the oocytes. These results suggest that (1) the cumulus cells surrounding the eggs were dispersed by sperm hyaluronidase, (2) hyaluronidase was inhibited by colominic acid and by sulfated colominic acid, (3) sulfated colominic acid inhibits sperm penetration of zona pellucida by the inhibition of hyaluronidase and/or some enzymes required for mouse gametes fertilization.
- 公益社団法人日本薬学会の論文
- 2000-08-01
著者
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OHTA Yasuhiro
Kyoto Research Laboratories, Marukin Shoyu Co. Ltd.
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Kawai Yuichi
Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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MIYAKE Masaharu
Faculty of Pharmaceutical Sciences, Kobe Gakuin University
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Kakehi Kazuaki
Faculty Of Pharmaceutical Sciences Kinki University
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ODA YASUO
Faculty of Pharmaceutical Sciences, Kinki University
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Kawai Yuichi
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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TAKEMOTO Mari
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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YAMAGUCHI Sinya
Kyoto Research Laboratories, Marukin Shoyu Co., Ltd.,
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Miyake Masaharu
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Takemoto Mari
Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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Ohata Y
Faculty Of Pharmaceutical Sciences Kinki University
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Oda Yasuo
Faculty Of Pharmaceutical Sciences Kinki University
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Yamaguchi Sinya
Kyoto Research Laboratories Marukin Shoyu Co. Ltd.
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Ohta Yasuhiro
Kyoto Research Laboratories Marukin Shoyu Co. Ltd
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