Evaluation of Fluorescence Polarization Method for Binding Study in Carbohydrate-Lectin Interaction
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概要
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The fluorescence polarization (FP) technique was evaluated to determine molecular interaction between plant lectins and polysaccharides, yeast cells and glycopeptide after labeling the lectins with fluorescein isothiocyanate. Use of Lycoris radiata agglutinin allowed determination of the molecular interactions with large biomolecules containing mannose oligomers and polymers. Another example using a fluorescein-labeled glycopeptide also indicated that use of the FP method would allow easy observation of the molecular interactions on the quantitative base. The present technique is highly sensitive and facile because it does not require any washing procedures before measurement.
- 公益社団法人日本薬学会の論文
- 1998-11-15
著者
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Kakehi Kazuaki
Faculty Of Pharmaceutical Sciences Kinki University
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Kakehi K
Kinki Univ. Higashi‐osaka Jpn
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ODA YASUO
Faculty of Pharmaceutical Sciences, Kinki University
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KINOSHITA Mitsuhiro
Faculty of Pharmaceutical Sciences, Kinki University
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NAKAYAMA Katsuyoshi
Faculty of Pharmaceutical Sciences, Kinki University
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Oda Yasuo
Faculty Of Pharmaceutical Sciences Kinki University
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Nakayama Katsuyoshi
Faculty Of Pharmaceutical Sciences Kinki University
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Kinoshita Mitsuhiro
Faculty Of Pharmaceutical Sciences Kinki University
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