Molecular Cloning and Nucleotide Sequence of Purine Nucleoside Phosphorylase and Uridine Phosphorylase Genes from Klebsiella sp.
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概要
- 論文の詳細を見る
Klebsiella sp. LF 1202 was isolated as a bacterium that can assimilate adenosine as a sole source of carbon and nitrogen [F. Ling et al. , Agric. Biol. Chem. , 55, 573-575 (1991)] from a soil sample. Both the purine nucleoside phosphorylase (PNPase) and uridine phosphorylase (UPase) of this bacterium were induced simultaneously when the bacterium was cultured in a medium containing adenosine or uridine as a sole source of carbon and nitrogen. This induction profile is different from that of Escherichia coli. Here we cloned and sequenced the gene corresponding to each enzyme. The open reading frame (ORF) of the PNPase gene consisted of 717 bp that encoded a polypeptide of 239 amino acids with a molecular weight of 26, 198. The ORF of the UPase gene consisted of 8Mbp that encoded a polypetide of 278 amino acids with a molecular weight of 28, 912.
- 社団法人日本農芸化学会の論文
- 1995-10-23
著者
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Inoue Y
Research Institute For Food Science Kyoto University
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Inoue Yoshiharu
Research Institute For Food S-cience Kyoto University
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Izawa Shingo
Research Institute For Food Science Kyoto University
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Inoue Yoshiharu
Research Institute For Food Science Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura A
Kyoto Univ. Kyoto
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Kimura Akira
Research Institute For Food Science Kyoto University
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Miki Takeo
The Inst. Of Enology And Viticulture Yamanashi Univ.
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Takehara Munenori
Research Institute for Food Science, Kyoto University
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Ling Feng
Research Institute for Food Science, Kyoto University
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Inoue Y
Kyoto Univ. Kyoto
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Kimura A
Research Institute For Food Science Kyoto University
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Ling Feng
Research Institute For Food Science Kyoto University
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Takehara Munenori
Research Institute For Food Science Kyoto University
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Miki T
The Institute Of Enology And Viticulture Yamanashi University
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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