Role of Glutathione Peroxidase against Oxidative Stress in Yeast Phenotypic Character of Lipid Hydroperoxide-Sensitive Mutants Derived from Hansenula mrakii
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概要
- 論文の詳細を見る
Wild type cells of Hansenula mrakii IFO 0895 were highly resistant to the oxidative stress caused by lipid hydroperoxide. The resistance was due to a glutathione peroxidase (GSHPx) which was induced when the yeast was cultured in a medium containing lipid hydroperoxide (Inoue, Y. et al., Agric. Biol. Chem., 54,3289-3293,1990). In order to investigate the role of GSHPx, two mutants sensitive to lipid hydroperoxide were isolated. The phenotypes of the mutants were temperature-dependent; i.e., the mutants could grow at 28℃, but not at 35℃ in the presence of lipid hydroperoxide. The mutants failed to induce the GSHPx at 35℃. However, the enzyme induced at 28℃ and prepared from both mutants was stable after incubation at 37℃ for 1 h.
- 社団法人日本生物工学会の論文
- 1993-03-25
著者
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Inoue Y
Research Institute For Food Science Kyoto University
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Inoue Yoshiharu
Research Institute For Food S-cience Kyoto University
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KIMURA AKIRA
Research Institute for Food Science, Kyoto University
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Kimura A
Kyoto Univ. Kyoto
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Kimura Akira
Research Institute For Food Science Kyoto University
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Inoue Y
Kyoto Univ. Kyoto
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Kimura A
Research Institute For Food Science Kyoto University
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TRAN LINH-THUOC
Research Institute for Food Science, Kyoto University
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Tran Linh-thuoc
Research Institute For Food Science Kyoto University
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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