A Bioluminescent Assay System for Whole-Cell Determination of Hormones
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概要
- 論文の詳細を見る
A bioluminescent-assay system was fabricated for an efficient determination of bioactive small molecules in physiological samples. The following three components were newly created for this assay system: (i) a single-chain probe exerting a 7.2-times stronger optical intensity than conventional ones, (ii) a high throughput assay device uniquely designed for the assay system with ca. one-fourth smaller standard deviation (SD) to samples than without the device, (iii) a buffer cocktail optimized for the assay system. The advantages of the assay system were evaluated by determining (i) the stress hormone levels in human saliva and (ii) multicolor imaging of genomic and nongenomic effects of woman sex hormones. This study guides on how to fabricate an efficient assay system for bioactive small molecules with convenience and high precision.
著者
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Kim Sung
Research Center, BOE TFT-LCD SBU, San 136-1, Ami-ri, Bubal-eub, Ichon-si, Gyeonggi-do 467-701, Korea
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Kimura Akira
Research Center for Solar Energy Chemistry, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
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Kimura Akira
Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)
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Suzuki Toshiyuki
IBEC center, National Institute of Advanced Industrial Science and Technology (AIST)
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