Solubilization, Purification, and Properties of Membrane-Bound D-Glucono-δ-lactone Hydrolase from Gluconobacter oxydans

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概要

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• Membrane-bound glucono-δ-lactonase (MGL) was purified to homogeneity from the membrane fraction of Gluconobacter oxydans IFO 3244. After solubilization with 1 M CaCl2, MGL was purified in the presence of Ca2+ and detergent. A single band corresponding to 60 kDa appeared in SDS–PAGE. The molecular weight of MGL was judged to be 120k. Differently from cytoplasmic lactonases, MGL showed optimum pH in an acidic range of 5–5.5. It was highly sensitive to metal-chelating agents such as EDTA, and the lost MGL activity was restored to the original level by the addition of divalent cations such as Ca2+ or Mg2+. The purified MGL was strictly dependent on Ca2+ and underwent rapid denaturing precipitation on Ca2+ depletion even in the presence of detergent. This communication can be the first one dealing with the solubilization, purification and properties of MGL.

• 社団法人 日本農芸化学会の論文
• 2009-01-23

著者

• 品川 恵美子

  宇部高専物質工学科

• MATSUSHITA Kazunobu

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ADACHI Osao

  Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Biological Chemistry, Faculty of Agricultre, Yamaguchi University

• SHINAGAWA Emiko

  Department of Chemical and biological Engineering, Ube National College of Technology

• Yakushi Toshiharu

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University

• Yakushi Toshiharu

  Dep. Of Biological Chemistry Fac. Of Agriculture Yamaguchi Univ.

• Murata K

  Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O

• Murata K

  Laboratory Of Basic And Applied Molecular Biotechnology Division Of Food Science And Biotechnology G

• Murata Kousaku

  Div. Of Food Sci. And Biotech. Grad. Sch. Of Agriculture Kyoto Univ.

• Adachi Osao

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Adachi Osao

  Department Of Agricultural Chemistry Yamaguchi University

• Shinagawa Emiko

  Department Of Chemical And Biological Engineering Ube National College Of Technology

• Matsushita Kazunobu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Matsushita Kazunobu

  Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University

• Murata Kousaku

  Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O

• Matsushita K

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Takaki Yoshihiro

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Yakushi Toshiharu

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• 松下 一信

  山口大 農

• Adachi O

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Ano Yoshitaka

  Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University

• Shinagawa Emiko

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• Matsushita Kazunobu

  Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University

• ANO Yoshitaka

  Department of Applied Bioscience, Faculty of Agriculture, Ehime University

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