Involvement of Multiple Copper/Topa Quinone-Contanining and Flavin-Containing Amine Oxidases and NAD(P)^+ Aldehyde Dehydrogenases in Amine Degradation by Filamentous Fungi
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概要
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Eighty-five different fungal strains were screened for the presence of amine oxidase after it was induced by n-butylamine, methylamine or spermine. Amine oxidase activity was detected in all Aspergillus, Penicillium, and most of Monascus strains, as well as in Pullularia, Doratomyces, Armillaria, Fusarium, Gibberella, Sporotricus, Trichophyton, Beauveria, Caldariomycesm, Helicostylum, and Trichoderma, but not in Mucor, Neurospora, Syncephalis, Keratinomyces, Eurotium, Zygorhynchus, or Cladosporium. By electrophoresis of native proteins with amine oxidase activity staining, two to three different enzymese were found in most of the strains. Predominant among these were copper-containing amine oxidases (EC 1,4,3,6), which were detected by Western blotting with polyclonal antibody against copper amine oxidaase from Aspergillus niger AKU 3302. On SDS-PAGE, most of the enzymese showed bands corresponding to a molecular mass of about 75 kDa. Enzymes that did not react with the antibody against copper amine oxidase did not react either with the antibody against flavin-containing monoamine oxidase from Micrococcus luteus. However, the enzymatic activities of extracts that contained non-coopper amine oxidases was substantially decreased by pargyline, an inhibitor specific to flavin-containing amine oxidases. Therefore, these enzymes are thought to belong to the class of flavin-containing amine oxidases (EC 1.4.3.4). Besides amine oxidase activity, increased aldehyde dehydrogenase activity was found in selected strains after induction with amines, from which it is supposed that amines are degraded via aldehydes to corresponding carboxylic acids. Two alternate metabolic pathways are suggested for methylamine and for large aromatic amines.
- 社団法人日本生物工学会の論文
- 1997-09-25
著者
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MATSUSHITA Kazunobu
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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ADACHI Osao
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Adachi Osao
Department Of Agricultural Chemistry Yamaguchi University
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Frebort I
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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FREBORT IVO
(Current address)Centre for Molecular Biology and Medicine, Department of Biochemistry, Faculty of S
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Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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