A Synthetic Medium for Bacterial Cellulose Production by Acetobacter xylinum subsp. sucrofermentans
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概要
- 論文の詳細を見る
Of several organic nitrogen sources tested, corn steep liquor (CSL) was found to be the most suitable for cellulose production by Acetobacter xyliuum subsp. sucrofermentans BPR 2001. When lactate which was detected only in CSL, was added to culture media containing other nitrogen sources, cellulose production was stimulated to levels similar to that in CSL medium. Lactate was found to stimulate cell growth during the early stage of culture and shown to function by linking with the respiratory chain and generating energy for growth. Therefore, it was speculated that lactate acts as an accelerator driving the TCA cycle as well as an energy producer, resulting in high cellulose production and rapid cell growth. The effects of various amino acids were also investigated: L-methionine was found to be essential for high cellulose yields and stimulation of cell growth during the eaily stage of culture. On the basis of these observations, a defined synthetic medium, containing lactate and methionine, was formulated, with which cellulose production of 90% of that with the CSL medium was reached in jar fermentor cultures.
- 社団法人日本農芸化学会の論文
- 1996-04-23
著者
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Adachi Osao
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita Kazunobu
Derartment Of Blological Chemistry Faculty Of Agriculture Yamaguchi University
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Matsushita K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Yoshinaga F
Bio-polymer Research Co. Ltd.
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Yoshinaga Fumihiro
Bio-polymer Research Co. Ltd.
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Tsuchida T
Bio-polymer Research Co. Ltd.
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Matsuoka Masanobu
Bio-polymer Research Co. Ltd. Ksp R&d Business Park Bldg.
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Tsuchida Takayasu
Central Research Laboratories Of Ajinomoto Co. Inc.
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Tsuchida Takayasu
Bio-polymer Research Co. Ltd.
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Tsuchida T
Bio-polymer Res. Co. Ltd.
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