Pyruvic Acid Production by an F_1-ATPase-defective Mutant of Escherichia coli W1485lip2
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概要
- 論文の詳細を見る
An F_1-ATPase-defective mutant, TBLA-1, was constructed by the transduction of a defective gene for the α subunit of F_1-ATPase, atpA401, into Escherichia coli W1485lip2, a lipoic acid-requiring pyruvic acid producer. The pyruvic acid production of the strain TBLA-1 was found to be improved markedly compared with that of strain W1485lip2. In cultures using a jar fermentor, the strain W1485lip2 consumed 50g/liter of glucose and produced 25g/liter of pyruvic acid after culture for 32 h, while strain TBLA-1 consumed the same amount of glucose, and produced more than 30g/liter of pyruvic acid in a 24-h culture. A revertant, No. 63-1, derived from the strain TBLA-1, had a normal level of F_1-ATPase activity, and showed a similar pattern of pyruvic acid production to that of strain W_1485lip2.
- 社団法人日本農芸化学会の論文
- 1994-12-23
著者
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Yokota Atsushi
Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University
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TOMITA FUSAO
Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University
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TAKAOKA NAOHISA
Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,
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Yokota A
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Yokota Atsushi
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Tomita Fusao
Laboratory Of Applied Microbiology
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Terasawa Yukio
Suntory Limited
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Shimizu Hideaki
Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,
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Yokota A
Hokkaido Univ. Hokkaido Jpn
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Terasawa Yukio
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Shimizu H
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Takaoka Naohisa
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Shimizu Hideaki
Laboratory For Structural Neuropathology Brain Science Institute Riken
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