Characterization of a Novel Gene Encoding a Putative Single Zinc-finger Protein, ZIM, Eepressed during the Reproductive Phase in Arabidopsis thaliana

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概要

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• By differential screening of an arrayed normalized cDNA library from the inflorescence apex in Arabidopsis, a cDNA clone having a deduced amino acid sequence with a motif for a zinc finger was isolated as one of the genes expressed specifically in the reproductive phase. The deduced protein has a modular structure with a putative single C2-C2 zinc-finger motif distantly related to a GATA-1-type finger, a basic region with a sequence resembling a nuclear localization signal, and an acidic region. The gene seemed to have been formed by the exon-shuffling during its molecular evolution, since individual domains are encoded by discrete exons. RNA gel blot analysis showed its expression in shoot apex and flowers in the reproductive phase. The gene was named ZIM for Zinc-finger protein expressed in Inflorescence Meristem. The nuclear localization of ZIM was detected using GFP as a reporter. These results suggest that ZIM is a putative transcription factor involved in inflorescence and flower development.

• 社団法人日本農芸化学会の論文
• 2000-07-23

著者

• Yokota A

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Yokota A

  Nara Inst. Of Sci. And Technol. (naist) Nara Jpn

• Yokota Akiho

  Plant Molecular Physiology Research Institute Of Innovative Technology For The Earth (rite)

• Yokota Akiho

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Yokota Akiho

  Plant Molecular Physiology Laboratory Research Institute Of Innovative Technology For Teh Earth (rit

• Takemura M

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Takemura Miho

  Grad. Sch. Of Biol. Sci. Nara Inst. Of Sci. And Tech.

• KOHCHI Takayuki

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Fujita H

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Kohchi T

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Kohchi T

  Laboratory Of Plant Molecular Biology Department Of Agricultural Chemistry Faculty Of Agriculture Ky

• Kohchi Takayuki

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Yokota A

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• FUJITA Hidetomo

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• TAKEMURA Miho

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• NISHII Akiko

  Graduate School of Biological Sciences, Nara Institue of Science and Technology

• SHIKATA Masahito

  Graduate School of Biological Sciences, Nara Institue of Science and Technology

• Nishii Akiko

  Graduate School Of Biological Sciences Nara Institue Of Science And Technology

• Shikata Masahito

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Fujita Hidetomo

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Yokota Akiho

  Graduate School Of Biological Science Nara Institute Of Science And Technology

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