L-Tryptophan Production by a Pyruvic Acid-Producing Escherichia coli Strain Carrying the Enterobacter aerogenes Tryptophanase Gene

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概要

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• Pyruvic acid-producing Escherichia coli W1485lip2 was transformed with the plasmid pKT901EA that carries the tryptophanase structural gene from Enterobacter aerogenes SM-18 downstream of the tac promoter. In the course of culture of the transformant in a pyruvic acid fermentation medium, isopropyl-β-D-thiogalac-topyranoside and other nutrients were supplied to the medium to induce tryptophanase expression. After 32-h culture, 28.6 g/l of pyruvic acid had been produced, and a large amount of tryptophanase had been accumulated in the cells. By addition of ammonia and indole to the culture broth, the enzymatic synthesis of L-tryptophan was then started. Our results showed that 23.7 g/l of L-tryptophan had been produced after reaction for 36 h (total 68 h).

• 社団法人日本生物工学会の論文
• 1996-12-25

著者

• Yokota Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• TOMITA FUSAO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• Yokota A

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Tomita Fusao

  Laboratory Of Applied Microbiology

• KAWASAKI KOSEI

  (Present address)Hokkaido National Industrial Research Institute

• Yokota A

  Hokkaido Univ. Hokkaido Jpn

• Kawasaki K

  Bioscience And Chemistry Division Hokkaido National Industrial Research Institute Aist

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