Molecular Cloning of the Gene Encoding the di-D-Fructofuranose 1,2':2,3' Dianhydride Hydrolysis Enzyme (DFA IIlase) from Arthrobacter sp. H65-7

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概要

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• The gene encoding an intracellular enzyme hydrolyzing di-D-fructofuranose 1,2': 2,3' dianhydride (DFA III)(DFA IIIase) was cloned from the genomic DNA of Arthrobacter sp. H65-7 for the first time. The single open reading frame (ORF) of the DFA IIIase gene consisted of 1368-bp encoding 455 amino acids. DFA IIIase showed a phylogenetically distinct position from other inulindegrading enzymes and showed similarity only with inulin fructotransferases (depolymerizing)(inulase II, EC 2.4.1.93) from Arthrobacter globiformis C11-1, Arthrobacter sp. A-6, and Arthrobacter sp. H65-7 (48.7-50.3%), and inulin fructotransferase (DFA I-producing)(EC 2.4.1.200) from A. glohiformis S14-3 (44.4%). An Escherichia coli transformant harboring a recombinant plasmid, pINB2, in which the DFA IIIase gene was fused with the β-galactosidase of pUC19 and under the control of the lac promoter, expressed DFA IIIase and the cloned enzyme produced inulobiose from DFA III similarly to the DFA IIIase of the wild-type strain, Arthrobacter sp. H65-7.

• 社団法人日本生物工学会の論文
• 2003-05-25

著者

• Yokota Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• TOMITA FUSAO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• SAITO Katsuichi

  Department of Upland Agriculture, National Agricultural Research Center for Hokkaido Region

• 冨田 房男

  北海道大学農学部

• 冨田 房男

  Hokkaido Study Center The University Of Air

• 横田 篤

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Yokota Atsushi

  Lab. Of Microbial Physiology Res. Fac. Of Agriculture Hokkaido Univ. Kita 9 Nishi 9 Kita-ku Sapporo

• 横田 篤

  北大 大学院農学研究院

• Yokota Atsushi

  Laboratory Of Bioorganic Chemistry

• Yokota A

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Yokota Atsushi

  Hokkaido Univ. Sapporo Jpn

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Tomita Fusao

  Laboratory Of Applied Microbiology

• Tomita Fusao

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Tomita Fusao

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• SUMITA Yoko

  Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture,

• Sumita Y

  Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci

• NAGASAKA YOSUKE

  Laboratory of Applied Microbiology, Research Group of Molecular Bioscience, Division of Applied bios

• Nagasaka Yosuke

  Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci

• Saito Katsuichi

  Department Of Upland Agriculture National Agricultural Research Center For Hokkaido Region

• Saito Katsuichi

  Memuro Upland Farming Research Station National Agricultural Research Center For Hokkaido Region

• Sumita Yoko

  Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci

• Yokota A

  Hokkaido Univ. Hokkaido Jpn

• Saito K

  Department Of Upland Agriculture National Agricultural Research Center For Hokkaido Region

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