Purification and Characterization of D-Sorbitol Dehydrogenase from Membrane of Gluconobacter suboxydans var. α
スポンサーリンク
概要
- 論文の詳細を見る
D-Sorbitol dehydrogenase was solubilized from the membrane fraction of Gluconobacter suboxydans var. α IFO 3254 by a procedure involving solubilization of the enzyme with Triton X-100 in the presence of KCl and D-sorbitol. Purification of the enzyme was performed by fractionation with polyethylene glycol 6000, and column chromatographies on DEAE- and CM-cellulose in the presence of Triton X-100. The enzyme was purified about 240-fold from the membrane fraction of the organism. The purified enzyme was tightly bound to a c-type cytochrome existing as a dehydrogenase-cytochrome complex. The dehydrogenase was found to be a flavoprotein, and its flavin moiety was covalently bound to the dehydrogenase protein. The optimum pH of the enzyme activity was 4.5, and the optimum temperature was 25°C. D-Sorbitol was specifically oxidized by the purified enzyme, and D-mannitol was also oxidized to 5% of the reaction rate with D-sorbitol.
- 社団法人 日本農芸化学会の論文
著者
-
Adachi Osao
Laboratory Of Applied Microbiology Department Of Biological Chemistry Faculty Of Agriculture Yamaguc
-
SHINAGAWA Emiko
Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Faculty of Agriculture, Ya
-
Ameyama Minoru
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Faculty Of Agriculture Yamag
-
Matsushita Kazunobu
Laboratory Of Applied Microbiology Department Of Biological Chemistry Faculty Of Agriculture Yamaguc
関連論文
- An Ideal Basal Medium for Assaying Growth Stimulating Activity of Pyrroloquinoline Quinone with Acetobacter aceti IFO 3284(Microbiology & Fermentation Industry)
- An Enzyme Catalyzing Liberation of Pyrroloquinoline Quinone (PQQ) from PQQ-Adducts(Microbiology & Fermentation Industry)
- Nitroblue Tetrazolium Reduction of Rice Leaf Slices Stimulated with Blast Fungus Hyphal Component(Pesticide Chemistry)
- Purification and Properties of 3α-Hydroxysteroid Dehydrogenase from Pseudomonas putida
- Effects of cycloheximide, actinomycin D and ethylene on the increase and subsequent decrease in acid invertase activity in wounded sweet potato
- Characterization of Quinohemoprotein Amine Dehydrogenase from Pseudomonas putida
- Some investigations on inactivation of phenylalanine ammonia-lyase in cut-injured sweet potato root tissue
- Purification and Properties of Methanol Dehydrogenase and Aldehyde Dehydrogenase from Methylobacillus glycogenes(Microbiology & Fermentation Industry)
- Crystallization and Properties of _L-Phenylalanine Ammonia-lyase from Rhodosporidium toruloides(Microbiology & Fermentation Industry)
- Calcium in Quinoprotein Methanol Dehydrogenase Can Be Replaced by Strontium(Microbiology & Fermentation Industry)
- Effect of Extracellular pH on the Respiratory Chain and Energetics of Gluconobacter suboxydans(Microbiology & Fermentation Industry)
- Crystallization and Properties of Amine Dehydrogenase from Pseudomonas sp. (Microbiology & Fermentation Industry)
- Preparation of Cells and Cytoplasmic Membranes of Acetic Acid Bacteria Which Exclusively Contain Quinoprotein Aldehyde Dehydrogenase(Microbiology & Fermentation Industry)
- Enzymatic Determination of Pyrroloquinoline Quinone with a Quinoprotein Glycerol Dehydrogenase(Microbiology & Fermentation Industry)
- Mammalian Choline Dehydrogenase Is a Quinoprotein
- Mode of Binding of Pyrroloquinoline Quinone to Apo-glucose Dehydrogenase
- Microbial Production of Pyrroloquinoline Quinone
- Crystalline NADP-Dependent D-Mannitol Dehydrogenase from Gluconobacter suboxydans
- Purification and Properties of 3α-Hydroxysteroid Dehydrogenase from Pseudomonas putida
- Isolation and Characterization of Outer and Cytoplasmic Membranes from Spheroplasts of Acetobacter aceti
- D-Glucose Dehydrogenase of Gluconobacter suboxydans: Solubilization, Purification and Characterization
- Crystallization and Characterization of a Novel Aminopeptidase from Trichosporon cutaneum
- Growth Stimulating Substance for Microorganisms Produced by Escherichia coli Causing the Reduction of the Lag Phase in Microbial Growth and Identity of the Substance with Pyrroloquinoline Quinone
- Crystallization of Membrane-bound Alcohol Dehydrogenase of Acetic Acid Bacteria
- Growth Stimulation of Microorganisms by Pyrroloquinoline Quinone
- How Many Times Should the Inoculum Cells be Rinsed before Inoculation in the Assay for Growth Stimulating Activity of Pyrroloquinoline Quinone?
- Purification and Characterization of the Quinoprotein D-Glucose Dehydrogenase Apoenzyme from Escherichia coli
- Purification and Characterization of D-Sorbitol Dehydrogenase from Membrane of Gluconobacter suboxydans var. α
- Purification and Characterization of 2-Keto-D-gluconate Dehydrogenase from Gluconobacter melanogenus
- Solubilization, Purification and Properties of Membrane-bound Glycerol Dehydrogenase from Gluconobacter industrius
- Purification and Characterization of Aldehyde Dehydrogenase of Acetobacter aceti